Expression Of Toll-like Receptor 4 And Nuclear Factor-kappa Bp65 In Peripheral Blood Lymphocytes And Monocytes From Patients With Psoriasis Vulgaris

Objective:To investigate the expression Toll-Iike receptor 4(TLR4)and Nuclear Factor-kappa B(NF-κBp65)in peripheral blood lymphocytes and monocytes from patients with psoriasis vulgaris and their roles in the pathogenesis of psoriasis.Methods: location by direct immunofluorescence, flow cytometry and immunocytochemistry were performed to measure the expressions of TLR4 and NF-κBp65 in peripheral blood lymphocytes and monocytes , respectively. Blood samples were obtained from 22 patients with psoriasis vulgaris and 15 normal controls.Results:TLR4 level was up regulated in the patients compared to controls ( P <0.05 ),and The expressions of TLR4 in peripheral blood lymphocytes and monocytes were significantly higher in patients with the active stage of psoriasis vulgaris than the stable stage(P<0.05). Although the expression of activated NF-κBp65 in peripheral blood in patients and in normal controls , patients with the active stage of psoriasis vulgaris and the stable stage are different ,no statistical difference was noticed(P>0.05).Conclusion:There is an abnormal expression of TLR4 in patients with psoriasis vulgaris suggesting that the TLR4 and the innate immune response mediated by it may be very important to the pathogenesis of psoriasis vulgaris, which may be through product -ing more pro-in?ammatory cytokines.