The Fabrication Of Spherical AFM Tip And Experimental Research Of Mechanical Properties Of Cancer Cells

Cancer has posed a powerful threat to human health in recent decades. The histopathological diagnosis and chemotherapy technology of the tumor is maturing; however, fundamental issues as the pathogenesis of cancer and metastatic mechanism stay vague. As the latest hot spot researching cancer cells in the single-cell level based AFM, the technology can not match advantage, but most focused on basic research, e.g. the AFM topography imaging. The method analyzing mechanical properties of cancer cells from a mechanical view has been limited by investigating way, which has been less reason for concern, are closely related with cancer cells metastasis. The paper aimed to research their mechanical properties and the impact of metastasis in the use of self-made spherical ball tip, based on the AFM nanoindentation features in the liquid environment.First, functional transformation of existing solid triangle cantilever probe tip used in solid namoindentation, extended the application of AFM in the biological field. By laboratory equipment existing, fabricated spherical tip fitting for nanoindentation of living cells in liquid by self, and calibrated its elastic constants by Cleveland method. The production process also applies to stick other micron ball to the micron spikes.Then, analyzed of all aspects of the conditions involved in the nanoindentation of living cells in the liquid environment, including the choice of living cells, culture, transport, liquid experiment environment to establish, what's more, the modification of the equipment, test improvement, operation optimization, and the specific calibration of the instrument before the experiment, etc., each of which relates to experiment can proceed smoothly. The summary of quick cell targeting in liquid indentation has good practicality and versatility.Finally, in the PBS liquid environment to maintain their best physical activity, experiments were performed between Anip-973 and AGZY-83a lung cancer cells with different metastatic ability. The results were implemented nonlinear fitting upon the stress-relaxation model derived from Hertz contact equation. ANOVA was conducted to determine the differences between each of the mechanical parameters, in order to examine its impact on cancer metastasis.