Genetic Analysis Of The Cytochrome P450 (CYP) 2J2 And Soluble Epoxide Hydrolase Genes In Chinese Subjects With Type 2 Diabetes

Background and Purpose—The association between common variants of CYP2J2 gene and EPHX2 gene with the development of cardiovascular diseases (CVD) have been predominantly identified in previous studies. However, this study within persons with type 2 diabetes mellitus (T2DM) evaluated whether single-nucleotide polymorphisms (SNPs) in the same genes could distinguish T2DM risk.Methods—Gene promoter region, all exons and 3`-untranslated region of the CYP2J2 and EPHX2 genes were directly sequenced in 48 individuals. Genotypings for the SNPs which were eligible for inclusion in the study were performed using TaqMan SNP Genotyping Assays in 1135 patients with T2DM and 1139 control subjects.Results—New of the most relevant polymorphisms in terms of frequency and functional importance with the CYP2J2 and EPHX2 were not found. Genotyping data indicate that no significant di?erence were observed in genotype distribution (χ2 test; genotype: p=0.717; p=0.304; p=0.322, respectively) and allele frequency (p =0.732; p=0.338; p=0.427, respectively) between Chinese non-diabetic subjects (n=1139) and type 2 diabetic patients (n=1135). Multivariate logistic analysis data indicate that no significant associations were found between rs890293, rs751141 or rs1126452 polymorphism variant allele and the development of T2DM under a dominant model a recessive model and an additive model after or not adjustment for conventional risk factors. Similarly, none of the polymorphisms was found to associate with insulin resistance under different genetic models between cases and controls (data not shown).Conclusion—The lack of associations between SNPs (rs890293, rs751141 or rs1126452) and T2DM investigated in the Chinese Han population suggests CYP2J2 gene and EPHX2 gene may be not associated with T2DM.