The Plasma And Tissue Expression Of Osteopontin And CD44v6 In Gastric Carcinoma And Their Significance

Objective: The aims of the present study are to detect the plasma and tissue expression of osteopontin (OPN) and CD44v6 in patients with gastric carcinoma , analyze the relationship between the expression and clinical pathological features, and illustrate their role in carcinogenesis and development.Methods: 40 cases of gastric carcinoma patients were collected from the Department of Gastrointestinal Surgery in Union Hospital during February 2010 to July 2010.All specimens were confirmed by pathology. There were 27 cases of males and 13 cases of females, aged from 24 to 76 years old, median age was 56 years old. None of them was done any pre-operative treatment for gastric cancer. 20 serums of healthy people in our hospital and 20 gastric mucosa specimens from 5 centimeters above the edge of carcinoma were collected for control group. The plasma levels of OPN and CD44v6 in 40 gastric carcinoma patients, 20 healthy subjects were measured by enzyme linked immunosorbent assay (ELISA). The tissue expression of OPN and CD44v6 were examined by immunohistochemical S-P method in 40 gastric carcinoma and 20 adjacent gastric mucosa tissues.Results: 1. Plasma levels and tissue expression of OPN in 40 gastric carcinoma patients were significantly higher than in the control groups(p=0.0087 and p=0.0021 respectively), and positively associated with histological grade,clinical stage and lymph node metastasis.2. Plasma levels and tissue expression of CD44v6 in 40 gastric carcinoma patients were significantly higher than in the control groups(p=0.0055 and p=0.0042 respectively), and positively associated with histological grade,clinical stage and lymph node metastasis.3. The plasma level of OPN in gastric cancer patients is positively correlated with that of CD44v6 (r=0.038, p<0.05).Conclusions: 1. Plasma levels and tissue expression of OPN in 40 gastric carcinoma patients were significantly higher than in the control group, and positively associated with histological grade,clinical stage and lymph node metastasis. The results showed that osteopontin was involved in tumorgenesis, could reflect the state of body of tumor burden and predict tumor progression.2. Plasma levels and tissue expression of CD44v6 in 40 gastric carcinoma patients were significantly higher than in the control group, and positively associated with histological grade,clinical stage and lymph node metastasis. These pointed out that CD44v6 was related to oncogenesis, expression in serum and tissue of CD44v6 could be biological indicators for dynamic observation of gastric cancer.3. The plasma level of OPN in gastric cancer patients is positively correlated with that of CD44v6, represent that they are synergetic in genesis and invasion of gastric cancer. Combined detection of serum levels of both may provide new biological basis for diagnosis of gastric cancer.