Effects Of Sleep Deprivation On The Expression Of Glucose Regulation Protein And Caspase-12 In The Rat Brain

To observe the influences of Sleep Deprivation(SD) on the expressions of GRP and caspase-12 on different brain regions. so as to investigate the possible effect of molecular chaperones and apoptosis factor during SD. To make rat model for SD of difference duration. To detect expressions of GRP78, GRP94 and caspase-12. To detect cell apoptosis. The transcription of GRP78 increased gradually on 1d and 3d after SD, no change on 5d and 7d after SD. The transcription of GRP94 had no improvement during SD. The transcription of GRP78 and GRP94 increased after revival sleep. Transcription of caspase-12 gradually increased during SD, no change after revival sleep. The expressions of GRP protein increased on 1d and 3d after SD in cortex and hippocampus, no improvement on 5d and 7d, no improvement in the medulla. Only expressions of GRP94 increased in the medulla after revival sleep. Active caspase-12 increased from 1d to 3d after SD. No active caspase-12 existed for the rest. those in the hippocampus region were the most obvious. apoptosis cells were the most in the hippocampus on 1d and 3d after SD. Endoplasmic reticulum (ER) switch on self-protection mechanism after the short time of sleep deprivation. In the cerebral cortex region there were strongly protective reactions. It possibly is one of injury mechanisms on brain initiating ER-related apoptosis pathway. The damage caused by sleep-deprivation was the most sensitive to the hippocampal region.