Research On The Influence Of Submerged And Nonsubmerged Implant Denture On The Expression Of Bone Sialoprotein In The Process Of Implant-Bone Interface Remodeling In Beagle Dogs

Nowadays,with the improvement of people,s living and the development of implant technology, more and more implant denture are chosen by patiens. Traditional submerged implant denture has been used widely in clinic.While nonsubmerged implant,a modified form with the advantages of only one operation needed,and appropriate functional stimulating after implantation contributing to the growth and remodeling of bone surrounding implant,gets increasingly, clinical application of this form increases gradually in recent years. Reports related to comparation of submerged and nonsubmerged implants were lack. The loading implant is supported by the implant-bone interface, and bite force which transferred from the implant denture to it effects the dynamic remodeling of the interface.Bone sialoprotein(BSP) is a major extracellular non-collagen matrix protein in the bone and associated with formation and remodeling of bone tissue. It was reported that the expression of BSP in the bone-implant interface was higher. But the mechanism of the effect on BSP by loading remains unknown. OBJECTIVE:To study the expression of bone sialoprotein (BSP) in the process of implant-bone interface remodeling after loading of submerged and nonsubmerged implant dentures.METHODS:8 adult beagle dogs were used to build the animal model. Submerged and nonsubmerged implants were implanted into the bilateral mandible. Fixed metal full crown was used to carry out the submerged and nonsubmerged implant dentur.The beagle dogs were sacrificed by steps after loading.Immunohistochemistry technique was used to observe the dynamic expression of bone sialoprotein in the implant-bone interface.RESULTS:In the group after loading of implant denture, the expression of BSP in the implant-bone interface enhanced apparently than in the group of the control of unloading. The gray level enhanced respectively from 89.89+0.38 to 118.26+2.58. 2 weeks after loading of implant denture, the expression of BSP in the interface was observed, but there was no statistical difference between experimental and control groups. 4 and 8 weeks after loading , the expression of BSP was obviously higher than that in the control group , and that reached peak at 8 weeks. It came down to the level of the control group after loading of 12 weeks. No obvious difference of the expression of BSP in the implant-bone interface was observed between the submerged and nonsubmerged implant denture.CONCLUSION: The mechanical stress which transferred from implant to the bone tissue around it after loading of the submerged and nonsubmerged implant denture can stimulate the expression of BSP in the implant-bone interface, promote the interface remodeling. Furthermore, there is no distinct difference in the implant-bone interface remodeling after the loading of submerged and nonsubmerged implant denture.