Preliminary Study Of The Pregnancy Outcome On The Murine With Chlamydia Trachomatis By Blockade Of Costimulatory Signal CD86

Objective1. It is the purpose of establishment the murine model to produce genital infection transvaginally with Chlamydia trachomatis F,and to explore chlamydial trachomatis (CT) infection transvaginally in a murine model and to observe pregnancy-related outcomes as a result of CT inoculation.2 To explore chlamydial trachomatis (CT) infection transvaginally in a murine model and to observe pregnancy-related outcomes as a result of CD86mAb treatment, to investigate the levels of Th-1 type and Th-2 type cytokine.MethodsIt is the purpose of establishment the murine model to produce genital infection transvaginally with Chlamydia trachomatis F, after the female murine were pregnant, The model group not to administration;and The treatment group was injected anti-mouse CD86(CD86mAb)100μg of PBS,the control group to was inoculated intravaginally with 50μl of SPG without CT。ELISA was applied to analysis the expression of the serum cytokine levels of Th1 ( IFN-γ),Th2 ( IL-4) at day 12 of gestation,In each group the embryo resorption of pregnancy ,embryo weight,or maternal weight increment was recordedResults1. the model group was inoculated intravaginally Chlamydia trachomatis F type strain was 17.35% in the embryo resorption rate of pregnancy, the control group was 6.86%,There was significant differences between the two groups. the treatment group was 17.35% in the embryo resorption rate of pregnancy, There was significant differences between the treatment group and the model group(P < 0.05),but there were no differences between the treatment group and control group (P > 0. 05).2. There were no differences between three groups in the rate of failed pregnancy, embryo weight and maternal weight increment.3. Th-1type cytokines(IFN-γ) of the model group was increased significantly than the control group, and the ratio of IL-4 /IFN-γwith the model group was significantly higher than the control group,and results in Th1-type immune bias.the treatment group reduced the embryo resorption rate of pregnancy and the level of Th1 type immune bias.Conclusions :1. We established animal models with genital infection by Chlamydia trachomatis serotype F via transvagina procedure, CD86mAb was used to assess the effection on pregnancy outcome and the mechanism of immunoregulation after the model mice were pregnant, so as to confirm that the animal models with genital infection by Chlamydia trachomatis serotype F via transvagina procedure is feasible..2. Chlamydia trachomatis serotype F intravaginal vaccination of mice can cause embryo resorption rate of pregnancy, but it was no significant influence of pregnancy failure rate,embryo weight,maternal weight incremen. The CD86mAb intervention reduced the embryo resorption rate of pregnancy.3. Mice injected with Chlamydia trachomatis serotype F via transvagina procedure can produce specific cellular and humoral immunity, The cytokines in vivo were drifted toTh1-type, Th1-type cytokines can hinder the development of early embryos, and cause raise of embryo resorption rate. The CD86mAb improve pregnancy outcomes by reducing the embryo resorption rate and the level of Th1-type immune bias.