| ObjectiveTo evaluate the protective effect and possible mechanism of additional L-carnitine supplement to neonates and rats with myocardial injury.MethodsPart1 A model of anoxia-reoxygenation mouse cardiocytes was built and classified into three groups:control group,L-carniting group,anoxia group in order to measure the level of CK-MB and SOD in cell supernatant.Part2 Twenty neonates with normal serum CK-MB and one hundred new-borns with abnormal serum CK-MB were enrolled in this experiment.The formal(Ⅴ) were given routine therapy(control group) for seven days.The latter were randomly divided into five groups(20 cases for each group) and were treated in five diffrernt ways for seven days :The patients of small dose group(Ⅰ) and large dose group(Ⅱ) were given L-carnitine by intravenous drip at dosage of 0.1g/kg and 0.15g/kg,qd×1;The patients of compound drug group(Ⅲ) were given L-carnitine 0.1g/kg and fructose 0.16g/kg by intravenous drip,qd×1;The patients of fructose group(Ⅳ) were given fructose 0.16g/kg by intravenous drip ;The patients of oral supplement group(Ⅵ) were given L-carnitine 0.1g/kg orally,qd×1.Before and after the treatment,serum MB isoenzyme of creatine kinase(CK-MB) was measured with immunosuppression and enzyme rate respectively,in the meantime,we dral blood spot from heel of those neonates(except compound drug group) to measure the level of serum acylcarnitine with the help of tandem mass spectrometry.ResultsPart 1:Neonatal rat cardiomyocytes were successfully cultured in vitro.Part 2:1,Stable descent rate of each group was:Ⅰ75.0%,Ⅱ85.0%,Ⅲ85.0%,Ⅳ45.0%,Ⅴ85.0%,Ⅵ75.0%(χ2=6.67,P<0.05),curative rate of each group wasⅠ95.0%,Ⅱ95.0%,Ⅲ95.0%,Ⅳ75.0%,Ⅵ90.0%(χ2=1.29,P>0.05).2,The serum level of CK-MB in each group at 7 days after birth has diclined:Ⅰ(97±43)%,Ⅱ(87±86)%,Ⅳ(70±63)%,Ⅲ(83±75)%,Ⅵ(54±42)%,Ⅴ(8±7)% compared with postnatal day 1(P<0.05).3,The serum level of CK-MB in each group at 7 days after birth were:Ⅰ(34.25±14.2)U/L,Ⅱ(34.95±18.6)U/L,Ⅳ(57.21±40.1)U/L,Ⅲ(37.05±13.7)U/L,Ⅴ(33.7±10.9)U/L,Ⅵ(37.2±16.1)U/L(P<0.05).4,There is a sustained rise of serum L-carnitine in both small and large dose group during seven days of treatment (I 39.85±11.5,102.55±65.64,189.9±50.9 ,Ⅱ43.2±13.8,162.85±99.6,197.45±107.3 )μmol/L compared with other groups(P<0.05),wherease ,there is a slow decline of serum L-carnitine level in oral group during treatment(Ⅵ36.33±7.2,34.51±7.5,29.11±5.9)μmol/L,none of them has a side effect.5,Those neonates in control group and fructose group have a consistent decrease in the level of serum L-carnitine during the treatment compared with small and large dose group(Ⅳ40.85±15.4,38.14±18.1,27.57±9.7,Ⅴ29.11±11.6,23.75±7.2,17.93±4.2)μmol/L(P<0.05),The magnitude of free serum L-carnitine reduction in fructose group,control group and oral group at 7 days after birth were(32±17)%,(31±19)%,(24±16)%( P<0.05).6,There is a positive correlation between the free serum level of L-carnitine and CK-MB. Conclusion:1,Additional L-carnitine supplement dose play a positive role in bring up the serum level of L-carnitine,especially those who were given L-carnitine by intravenous drip.2,L-carnitine takes a better curative effect in bringing down the abnormal serum level of CK-MB compared with fructose.3,There is a positive correlation between the free serum level of L-carnitine and CK-MB. |
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