Expression And Significance Of S100A4, Survivin And COX-2 In Pancreatic Carcinoma Tissues

Objective:To detect the expression of calcium-binding protein S100A4, Survivin and COX-2 in pancreatic carcinoma (PC) and their correlation with clinicopathological parameters and prognosis of pancreatic carcinoma patients, investigate the mechanism of action of protein S100A4 in PC, to provide academic and experimental evidence of the treatment of PC by the intervention of S100A4.Methods:①he expressions of S100A4, Survivin and COX-2 were examined in 63 surgical specimens of primary pancreatic carcinoma and 11 surgical specimens of non-pancreatic carcinoma with immunohistochemistry PV method.②The relationship between S100A4, Survivin, COX-2, clinicopathological parameters and prognosis of PC was analyzed by X2 test in 63 cases of PC. Kaplan-Meier and multivariate Cox proportional hazards regression model analysis were applied to compare the survival curves and screen the independent prognosis factors respectively.Results:①Forty-seven of 63(74.6%)specimens of PC were positive for S100A4, Forty-four of 63(69.8%)pancreatic carcinoma specimens showed positive expressions of Survivin and Forty-six of 63(73.0%)specimens of PC were positive for COX-2 according to immunohistochemistry detection.②he positive expressions of S100A4 and Survivin were significantly correlated with PC (r= 0.570, P=0.000), The correlation was significant between S100A4 and COX-2 in PC (r=0.385, P=0.002), The positive expressions of Survivin and COX-2 were also significantly correlated with PC (r=0.613, P=0.000).③he expressions of S100A4 significantly correlated with TNM stages (P=0.002), lymph node metastasis (P=0.002)and distant metastasis (P=0.007); The expression of Survivin had a significant correlation with TNM stages(P=0.034), lymph node metastasis (P=0.020)and differentiation(P=0.000); The expressions of COX-2 significantly correlated with turner size (P=0.013), differentiation (P=0.001), TNM stages (P=0.021), lymph node metastasis (P=0.022) and distant metastasis (P=0.031).④he median survival time(>36 months) of 11 patients with S100A4(-)/Survivin (-) was significantly longer than that of the 37 patients with S100A4(+)/Survivin(+) (9 months, x 2=17.754, P=0.000); The median survival time(>36 months) of 8 patients with S100A4(-)/COX-2 (-) was significantly longer than that of the 37 patients with S100A4(+)/COX-2(+) (9 months, x 2=44.969, P=0.000); The median survival time(>36 months) of 9 patients with Survivin (-)/COX-2 (-) was significantly longer than that of the 38 patients with S100A4(+)/COX-2(+)(9 months, x 2=34.128, P=0.000).⑤The differentiation(P=0.002), TNM stages (P=0.002), S100A4(+)Expression (P=0.001), Survivin (+) expression(P=0.024) and COX-2(+) Expression (P=0.002) were the independent prognostic factors for pancreatic cancer.Conclusion:This study indicated that the expression of S100A4, Survivin and COX-2 proteins were related with clinicopathological parameters of pancreatic cancer patients, S100A4 combined with Survivin and COX-2 could cooperatively inhibit the cells into apoptosis, promote the proliferation and angiogensis in the process of growth and invasion in pancreatic cancer, over-expressions of S100A4, Survivin and COX-2 proteins maybe predict the poor prognostic of pancreatic cancer patients, They may be the potential indicators of metastasis and prognosis in pancreatic cancer. S100A4 expression in PC tissiue is related with the inhibition of cells apoptosis, promotion of the proliferation and angiogensis, S100A4 may be a new target for the treatment of PC, to provide academic and experimental evidence of the treatment of PC by the intervention of S100A4.