Experimental Study Of The Effects Of Humam Amniotic Membrane On Graft Bone Of Alveolar Cavity

Objective:The effects of human amniotic membrane in the autologous bone grafts for alveolar cavity healing was studied with mature animals in order to provided the basis for clinical.Methods:Group A randomly selected six from twelve three-month old local white rabbits were pulled out the right maxillary first and second premolar on general anesthesia, the alveolar cavities were covered with gingiva.Then these rabbits were divided randomly into group B and C, pulled out the left maxillary first and second premolar on general anesthesia, the alveolar cavities of Group B were transplanted autologous rib and covered with gingiva, Group C were transplant autologous ribs and covered with human amniotic membrane and gingival.The bilateral maxillary operations were done at one time, All process was accomplished by one surgeon. All specimen were evaluated by observation, X-ray test and histological analysis 12 weeks postoperative, statistical analysis the reconstruction height of the alveolar and the area percentage of the new bone.Result:Alveolar bone density of group C is higher than goop B and A by X-ray, and alveolar ridge of groop C is glossy and continuous. The reconstruction height of three groups alveolar bone is different, significant difference was found in the statistics(P< 0.05). The reconstruction height of the alveolar bone groop C<B< A. Alveolar bone healing of group C was better than group A and B by histology observation,new bone was found in three groups alveolar cavity,significant differences was found in the area percentage of the new bone (P< 0.05), new bone mass C>B>A.Conclusion:In the repairing rabbit's alveolar cavity with autologous bone grafts, human amniotic membrane can guide bone regeneration in using it to cover the surface of bone grafts, reduce bone grafts resorption and protect alveolar bone mass.