The Effects Of Rabbit Mandibular Defect Reconstruction Using Human Acellular Amniotic Membrane Loaded Bone Morphogenetic Protein-2 Microspheres

BackgroundBone defect is a common clinical problem.The treatment of bone defect includes autogenous bone grafting,allogeneic bone grafting and xenogeneic bone grafting.But bone reaorption and donor site complication must be considered.Rejection reaction and infection are also the disadvantages of this treatment.The tissue engineering bone has not been widely used in clinic.It is still at the experimental stage.Last century dentists said that bone defect can be treated by osteoinductive membrane.But research turn out that exposure of material,nondegradable material and difficulty in production are the obstacles of this technique.We propose that using human acellular amniotic membrane loaded bone morphogenetic protein-2 microspheres as a martrix of osteoinductive membrane.It is easy to gain and make HAAM,and this membrane has many biological characteristics which make it an ideal biological dressing and martrix.It has been widely applied in tissue reconstruction.HAAM loaded bone morphogenetic protein-2 microspheres can improve the ability of osteogenic induction which make it a good choice in bone defect recons tract ion.The purpose of this study is to make a new osteoinductive martrix using human acellular amniotic membrane loaded bone morphogenetic protein-2 microspheres.We' re going to research its ability of osteogenic induction combined with BMSCs.Therefore we can finally find a new way to treat mandibular bone defect and other bone defects.Objective and MethodsWe introduced a way to decellularize human amniotic membrane.HE staining showed that the HAM is completely decellularized.We used scanning electron microscope to see the structure of HAAM.We made bone morphogenetic protein-2 microspheres and researched its structure,drug loading capacity and sustained-release curve.We used whole bone marrow culture method to culture rabbit BMSCs,and researched its ability of osteogenic differentiation and adipogenic differentiation.And the BMSCs had been cultured with HAAM martrix.HE staining and SEM were made to research its structure.Animal experiment was made to research the osteoinductive ability of HAAM loaded bone morphogenetic protein-2 microspheres.ResultsThe HAAM was completely decellularized and it has a three dimensional network structure.Gelatin-agar microspheres has sustained-release ability and its shape was all round and almost the same size.The BMSCs has the ability of osteogenic differentiation and adipogenic differentiation.The martrix of HAAM loaded bone morphogenetic protein-2 microspheres can promote the bone defect reconstraction.ConclusionHAAM has good cell compatibility and can promote the growth of cells.The BMSCs are ideal seed cells of bone engineering.The martrix of HAAM loaded bone morphogenetic protein-2 microspheres combined with BMSCs can promote the bone defect reconstraction.