| Objective and Background:The salivary gland polymorphic adenomas are a seedling source in the epidermis pathological change's benign tumor, because of its pathology shape multiplicity, contains the epidermis, the mucilage and the chondroid ingredient richly, also calls the mixed tumor. Although it is one kind of benign tumor, but actually has certain attack and the recurrence, so WHO decides as it the critical lump. Recurs many times or the course elder becomes the malignant polymorphic adenomas hatefully. The salivary gland polymorphic adenoma's occurrences, become malignant are the multi-factors, the polygene variation comprehensive pathological change process. The research discovered that the cytogenetics change, the cancer gene, damps the cancer gene, to adhere molecular and the extracellular matrix protein, in the salivary gland polymorphic adenomas become malignant in the process to have certain function. The present mainly treatment is the surgery excises. PTEN was found in 1997 by 3 independent research team in American, a new tumor suppressor gene which discovered in the human 10th chromosome, until now is 1st which discovered has the specificity phosphoric acid enzyme activity to damp the cancer gene, this gene sudden change deactivation and human many kinds of malignant tumor occurrence development close related, in apoptosis, in the cell cycle and the cell migration process the key role. This experiment inquires into PTEN the gene to the salivary gland polymorphic adenoma occurrences in the gene level function, provides a new mentality for the salivary gland polymorphic adenoma's gene level's treatment, and lays the foundation for the salivary gland polymorphic adenoma clinical pharmacological treatment.Materials and Method:Take the patients in our hospital from January 2009 to December 2010, surgery and confirmed by pathological examination of specimens of 30 cases. Divided into A, B, C three groups, through immunohistochemical study PTEN gene in saliva auricular gland polymorphic adenomas, nearby salivary gland polymorphic adenoma lumps in 1cm organization and hypoglossis glandular tissue's expression. Between discusses the PTEN gene organizes and the salivary gland Normal tissue's expression relevance research in the salivary gland polymorphic adenomas and nearby the salivary gland polymorphic adenoma lumps, correlation analysis for data, application SPSS 17.0 statistical software for statistical analysis.Immunohistochemistry:Salivary gland polymorphic adenoma, polymorphic adenoma lcm tumor adjacent tissue, the sublingual gland tissue samples fixed, embedded and sliced; heat pressure cooker with citric acid solution repair repaired. Explains PTEN according to the reagent box operation the immunohistochemical dyeing, PTEN positive signal was yellow, brown yellow or brown granules, localized in the nucleus. PTEN positive reaction was yellow, brown yellow or brown granules, localized in the nucleus, each of 10 randomly selected cases of high power field, calculate the percentage of masculine cell. The masculine cell≥10% are the masculine case, masculine cell<10% negative cases according to treatment..Result:PTEN gene in salivary gland polymorphic adenomas positive for the expression of 18 cases, polymorphic adenomas lcm tumor tissue adjacent group of 25 patients with positive expression, the expression of the sublingual gland of group 28 cases were positive.1. Salivary gland polymorphic adenoma and polymorphic adenoma of salivary gland tumor tissue adjacent to lcm difference between two groups was statistically significant (P<0.05);2. Salivary gland polymorphic adenoma and the sublingual gland the difference was statistically significant (P<0.05);3. Polymorphic adenoma lcm tumor tissue adjacent to the sublingual gland with no significant difference between the two groups (P> 0.05).Conclusions:Through the immunohistochemical examination's statistics analysis, the PTEN gene's masculine expression has the inhibitory action to the salivary gland polymorphic adenoma's occurrences. |
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