The Expression And Effect Of GRP78 On Biological Behavior Of Hepatocellular Carcinoma With Different Metastatic Potentials

AIM:To explore the expression of GRP78 in hepatocellular carcinoma with different metastatic potentials. Study the effect of GRP78 on biological of hepatoce-llular carcinoma with different metastatic potentials after transfected by GRP78 ASODN.METHODS:(1) RT-PCR was used to analyzed the GRP78 mRNA expression in different hepatocellular carcinoma cell lines. (2) The GRP78 antisense oligonucleotides (GRP78 ASODN) was transfected into the two type hepatocellular carcinoma cell lines, HepG2 and MHCC97-H. RT-PCR was used again to assess the GRP78 mRNA expression in hepatocellular carcinoma cell lines. Transwell assay was used to detect hepatocellular carcinoma cell invasion and migration. Cell adhesion assay was employed to investigate hepatocellular carcinoma cell adhesion.RESULTS:The result of RT-PCR showed that both of the two type hepatocellular carcinoma cell lines expressed GRP78, but the expression of GRP78 in MHCC97-H was higher than HepG2(P<0.05). GRP78 mRNA was depressed effectly by GRP78ASODN in both hepatocellular carcinoma cell lines. The invasive, migratory and adhesive potentials of MHCC97-H after transfected by GRP78ASODN were significantly depressed than other groups (P<0.05). But there was no significant difference in HepG2 after transfeted by GRP78 ASODN (P>0.05)CONCLUSION:(1) The GRP78 was expressed in both of the hepatocellular carcinoma with different metastatic potentials.(2) Deprssed the expression of GRP78mRNA in different hepatocellular carcinoma cell lines after transfected by GRP78ASODN could inhibit the cell invasion, migration and adhesion. Depression the GRP78 of hepatocellular carcinoma cells could become a new way of molecular biology for hepatocellular carcinoma treatment. It is supposed that GRP78 may be a prospective molecule therapy target in hepatocellular carcinoma.