Preparation Of Coollagen Gel And Chitosan Gene Carrier

This paper provides prerequisite preparation for gene therapy of Collagen gel containing chitosan gene.The method of enzyme digestion was used to extract collagen from bovine tendon so that triple helix of collagen molecules can be maintained. And the extracted collagen was characterized. The extracted collagen viscosity and solid content were measured. In pepsin digest method, the best ratio for enzymes and material is that E to B equals 1 to 10. The extracted protein was confirmed to be collagen by FTIR and UV. At the same time, collagen gel was prepared by the extracted collagen, and collagen gel solid content was measured. The cell was seeded in collagen gel. The cell growth state was observed by optical microscope and the results show that the collagen gel can be used as 3D cell scaffold.In addition, a series of biology characterizations of natural chitosan were made. The results of gel retardation experiment indicated the chitosan had strong affinity to DNA. CS-DNA could form composite nano-particles with diameters from 100 to 300 nm and the surface potential is stable. The cytotoxicity was measured by MTT experiment that the results showed chitosan had no cytotoxicity and proved that chitosan can be used as gene carrier.