| Objictive: High incidence,high morbidity and mortality of cerebral infarction brought a heavy burden to the socity and the family, this study was to investigate the relationship between the serum level of matrixtalloproteinase-2, the volum size of infarction, degree of neurofunction impairment as well as prognosis in patients with acute cerebral infarction. We expect new intervention targets for clinical prevention and treatment of cerebral infarction.Methods:All cases were persons with health check and patients with acute ischemic stroke admitted to the medical department of Neurology of Heze Municipal Hospital since December 1,2009-September 30,2010.All cases were divided into A, cerebral infarction group:56 patients with acute ischemic strok treated in our hospital,aged from 35 years to 80 years,average.All were in line with the Fourth National Conference revised diagnostic criteria of cerebral infarction, and confirmed by brain CT or MRI. Hospital treatment closely follows the "china guideline to Cerebral Vascular Disease". According to the head CT or MRI lesion size, infarct volume by Pullicino formula (length×width×number of layers÷2) calculation, the cerebral infarction patients were divided into large groups (LI) 20 cases, lesion volume> 10cm3;middle infarction group (MI) 18 patients, lesion size (5-10cm3); small infarct group (SI) 18 cases, lesion size<5cm3. All cases were rated by the clinical neurological deficit scoring (CNFDS), developed based on the 1995 Fourth National Conference on cerebrovascular disease,then divided into severe group (31-45 points),17 cases, medium group(16-30 points),20 cases, light group (0-15 points), 19 cases. According to the National Institutes of Health Stroke Scale (NIHSS), the emerging infarction,which score at 7 days after admission compared with the time of admission increased by at least 2 points, parallel head CT excluded hemorrhagic infarction and other vascular diseases caused by is defined as the early neural function deterioration. The cerebral infarction group was divided into the early neurological deterioration in 26 patients, the non-early neurological deterioration group of 30 patients,which rate reduced at day 7 after admission compared with the admission; B control group:Select the normal healthy subjects, at the same period,as control group,30 cases, aged 42-70 years, mean 52.1±13.4 years. Fasting venous blood were taken 4ml,for two samples, cerebral infarction group were taken after the onset of 24h and 7d,contral group were taken at the first day and the 7d.The serum were collected afterone blood sample at room temperature to be solidified,and centrifugated for 10 minutes at 2000 r/min,then detected the MMP-2 concentration,the other sample was send to our biochemistry laboratory to tested the indicators of blood glucose, cholesterol, triglycerides, low density lipoprotein and lipoprotein a. Experimental data analyzed by statistical software SPSS11.5. Comparison between two groups were the number of samples t test, multiple samples mean comparision used to single-factor analysis of variance.Results:1. Compared with the control group, the systolic pressure, diastolic pressure, blood glucose, cholesterol,and lipoproteins of infarction group(onset 24-48h) were significantly different (P<0.01); Other indicators, including age, gender, coronary heart disease, cerebrovascular disease, family history, smoking, alcohol consumption, triglycerides were not significantly different between the two groups.2. Compared with the control group, cerebral infarction serum levels of MMP-2-in the course of 24h, at 7d were significantly higher (P<0.01);it was more higher at 7d than that of 24h(P<0.01).3. Of patients with cerebral infarcton,the MMP-2 level was positively correlated with the size of the infarction volum(P<0.05), the larger the infarction volum,the higer the serum level of mmp-2.4. Of patients with cerebral infarction,the MMP-2 level was positively correlated with neurologic dificit extent(P<0.01);i.e., the more severe neurologic impairment the higher the serum levels of MMP-2.5. The mmp-2 levels in patients with cerebral infarction correlated with different recent prognosis,i.e.,the higher the level of serum mmp-2, the worse the prognosis of patients with cerebral infarction.Conclusion: The results showed that the increased levels of MMP-2 of patients with cerebral infarction was positively correlated with the lesion size and severity, also closely correlated with prognosis.sugesting MMP-2 play an important role in the pathegenesis and development of acute ischemic cerebrovascular disease.earl detection of serum levels of MMP-2 could be a reliable indicator to determined the infarction size, severity and prognosis of patients. To reduce the level of MMP-2 may be effective for the treatment of cerebral infarction. |
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