Explore The Effects And Mechinisms Of Jingtongning Pill Which Treat And Relieve Cervical Spondylotic Vertebral Arteriopathy

Objective:To explore the effects and mechinisms of Jingtongning-pill (JTNP), which has effects on cervical spondylotic vertebral arteriopathy(CSA).Methods:70 healthy New Zealander rabbits in half respectively male and female,weighing 2.5kg to 3.Okg,were numbered.All rabbits were measured the mean velocity (Vm) in vertebral arteries(VA) and basilar arteries(BA) using transcranial doppler(TCD).Then all rabbits were injected tissue sclerosing liquid into the soft tissue on the left side of cervical vertebral transverse protuberance to set up the CSA models.Measured the Vm in left VA,right VA and BA using TCD once more to confirm the successful models.Next,Selected 40 successful model rabbits and grouped them into 5 groups randomly,8 in each group.All groups were administered of relevant drugs for 4 weeks,once a day.The positive group was administered Jingfukang-granule1.0g/kg.JTNP was administrated to the high-dose group 2g/kg,the middle-dose group 1g/kg,the low-dose group 0.5g/kg,respectively. The model control group was given same amount of saline. The normal group was composed of another 8 healthy rabbits and was fed on normal diet.27days after first administration,all rabbits were measured the mean velocities in left VA,right VA and BA using TCD.Next day,tested the content of ET,IL-1β,TNF-αin plasma,NO in serum,and observed the histopathological change of the injection tissue and brain by light microscope about histomorphology,cytoarchitecture,hyperplasia of connective tissue, increaseing of inflammatory cells,blood stagnation and so on.Besides,50 mouses were randomly signed into 5 groups,10 each group.The positive control group was administered of aspirin0.3g/kg.JTNP was administered to the high-dose group 5.4g/kg,the middle-dose group 2.7g/kg,the low-dose group 1.35g/kg,respectively.The normal control group was given same amount of saline,all groups were administered for a week.Half an hour after the last administration,all groups were injected acetic acid into belly and then observed the amount of body twisting in 15 minutes.Results:1.The detecting results by TCD show that after administration the mean velocities in left VA, right VA and BA were faster than the mean veloci-ties before administration.The mean velocities in left VA, right VA and BA of the high-dose group were very different from the mean velocities in left VA, right VA and BA of the model control group(P<0.01,P<0.01,P＜0.05,P<0.01).The mean velocities in left VA and right VA of the middle-dose group were also different from the mean velocities before administration(P<0.05).After administration, the mean velocities in left VA, right VA and BA were faster than the mean velocities in left VA and right VA of the model control group,the mean velocities in left VA, right VA and BA of the high-dose group were very different from the mean velocities in left VA and right VA of the model control group(P<0.01,P<0.05,P<0.01).The Vm in left VA of the middle-dose group was also different from the Vm in left VA of the model control group(P<0.05).In the high-dose group and the middle-dose group,the content of NO in serum was obvious higher than the model control group(P<0.01,P<0.05).In the high-dose group and the middle-dose group,the ET content in plasma was obvious lower than the model control group(?<0.01,P<0.05).In the high-dose group and the middle-dose group,the content of IL-lβin plasma was obvious lower than the model control group (P<0.05). In the high-dose group and the middle-dose group,the content of TNF-a in plasma was obvious lower than the model control group(P<0.01,P<0.05).By light microscope the observed results showed that the phenomenons,such as histomorphology,Cytoarchitecture, hyperplasia of connective tissue,increasing of inflammatory cells,blood stagnation and so on,of all tested groups were superior to the model control group. 2.JTNP analgesia study showed that the times of body twisting of the high-dose group and the middle-dose group were less than the model control group (P<0.05).Conclusion:JTNP has obvious therapeutic effects on cervical spondylotic vertebral arteriopathy modal rabbits.The mechenisms may be relateimproving the blood circulation, adjusting vessels,restraining inflammatory factors, boosting body ability of resistance pains and so on.