Expressions And Clinical Significance Of Annexinâ…¡,S100A4 And VEGF-C In Papillary Thyroid Carcinoma

Objective:Tumor development, invasion and metastasis are extremely complex processes of multi-factors, multi-step. Annexinâ…¡is a calcium-mediated phospholipid binding protein family, participate in a series depends on the calcium ion membrane biological functions. Annexinâ…¡can accelerate DNA replication and regulate cell cycle to promote tumor cell proliferation, through participation in fibrinolysis, cell adhesion to promote tumor metastasis, play an important role in tumor occurrence development,invasion and metastasis. S100A4 protein is a calcium binding protein, can through several aspects such as increase the exercise capacity of tumor cells, reduce the adhesion between tumor cells and promote angiogenesis, promote extracellular matrix degradation and remodeling to promote tumor metastasis. Vascular endothelial growth factor C is a new members of human vascular endothelial growth factor family, also known as lymphatic endothelial growth factor.It through binding receptor VEGFR-2, VEGFR-3 to autophosphorylation and stimulate the proliferation of lymphatic endothelial cells and regulate tumor angiogenesis, promote tumor cells into the lymphatic and spread to the body lymphatic system and transfer. In this study, the immunohistochemistry and in situ hybridization technique was used to test the different expressions of calcium phospholipid binding proteinâ…¡(Annexinâ…¡), calcium-binding protein S100A4 and vascular endothelial growth factor C (VEGF-C) proteins and Annexinâ…¡mRNA in tissues of 50 papillary thyroid carcinoma,14 thyroid adenoma,26nodular goiter and 10 normal thyroid gland,and investigate its relationship with the development of thyroid papillary carcinoma and lymph node metastasis, and the relevance of the three.Method:The immunohistochemistry S-P and in situ hybridization technique was used to test the different expressions of Annexinâ…¡,S100A4, VEGF-C proteins and Annexinâ…¡mRNA in tissues of 50 papillary thyroid carcinoma,14 thyroid adenoma, 26nodular goiter and 10 normal thyroid gland.Results:1. The positive expression rate of Annexinâ…¡protein in papillary thyroid carcinoma tissues was 82.00%(41/50), in hyroid adenoma tissues was14.29%(2/14) and in nodular goiter tissues was7.69%(2/26);whilewas negative in all of the normal thyroid tissues. The positive expression rate in papillary thyroid carcinoma tissues was higher than that in hyroid adenoma tissues (P<0.05), the positive expression rate was higher than that in nodular goiter.Positive rate of Annexinâ…¡were 100% (21/21) in lymph node positive groupand 68.97%(20/29) in lymph node negative group.There was significant difference between the 2groups (P<0.05). Annexin II expression was not correlated with sex,age and carcinoma size.2. The positive expression rate of S100A4 protein in papillary thyroid carcinoma tissues was 78.00% (39/50), in hyroid adenoma tissues was28.57 (4/14) and in nodular goiter tissues was3.85%(1/26);whilewas negative in all of the normal thyroid tissues. The positive expression rate in papillary thyroid carcinoma tissues was higher than that in hyroid adenoma tissues (P<0.05), the positive expression rate was higher than that in nodular goiter.Positive rate of S100A4 were95.24%(20/21) in lymph node positive groupand 65.52%(19/29) in lymph node negative group.There was significant difference between the 2groups (P<0.05). S100A4 expression was not correlated with sex,age and carcinoma size.3. The positive expression rate of VEGF-C protein in papillary thyroid carcinoma tissues was 76.00%(38/50), in hyroid adenoma tissues was35.71%(5/14) and in nodular goiter tissues was7.69%(2/26);whilewas negative in all of the normal thyroid tissues. The positive expression rate in papillary thyroid carcinoma tissues was higher than that in hyroid adenoma tissues (P<0.05), the positive expression rate was higher than that in nodular goiter.Positive rate of VEGF-C were95.24%(20/21) in lymph node positive groupand 62.07%(18/29) in lymph node negative group.There was significant difference between the 2groups (P<0.05) VEGF-C expression was not correlated with sex,age and carcinoma size.4. The positive expression rate of Annexinâ…¡mRNA protein in papillary thyroid carcinoma tissues was 74.00%(37/50), in hyroid adenoma tissues was14.29%(2/14) and in nodular goiter tissues was3.85%(1/26);while was negative in all of the normal thyroid tissues. The positive expression rate in papillary thyroid carcinoma tissues was higher than that in hyroid adenoma tissues (P<0.05), the positive expression rate was higher than that in nodular goiter.Positive rate of Annexinâ…¡mRNAwere90.48%(19/21) in lymph node positive groupand 62.07%(18/29)in lymph node negative group.There was significant difference between the 2groups (P<0. 05). Annexinâ…¡mRNA expression was not correlated with sex,age and carcinoma size.5. In papillary thyroid carcinoma, the expressions of Annexinâ…¡protein and S100A4, VEGF-C proteins were positively correlated; the expressions of S100A4 protein and VEGF-C protein were positively correlated(P< 0.05); the expressions of Annexinâ…¡protein and Annexinâ…¡mRNA were positively correlated.Conclusion:1. Annexinâ…¡protein, S100A4 protein and VEGF-C protein in normal thyroid tissues, nodular goiter tissues and hyroid adenoma tissues is no expression or low expresseion,but in papillary thyroid carcinoma tissues is high expression,it implies that Annexinâ…¡protein, S100A4 protein and VEGF-C protein related to the occurrence of papillary thyroid carcinoma.2. The positive expression rate of Annexinâ…¡protein, S100A4 protein and VEGF-C protein in the lymph node metastasis group were higher than that in nonlymph node metastasis group, respectively,a significant difference between them,which indicate that the high expression of Annexinâ…¡protein, S100A4 protein and VEGF-C protein may promote the invasion and metastasis of papillary thyroid carcinoma.3. The expressions of Annexinâ…¡protein and Annexinâ…¡mRNA were positively correlated,and the positive expression rate of Annexin II protein was higher than Annexinâ…¡mRNA in papillary thyroid carcinoma, which indicate that the high expression of Annexin II protein have a positive correalation with the high transcription activity of mRNA.4. In papillary thyroid carcinoma,the expression of Annexinâ…¡protein, S100A4 protein,VEGF-C protein and Annexinâ…¡mRNA was positively correlated,it implies they have certain synergy to promote invasion and lymph node metastasis of papillary thyroid carcinoma,participate in tumor invasion and metastasis.