| ObjectiveTo explore the expressions of NS and TBX2, and investigate their correlations to the infiltration, metastasis of papillary thyroid carcinomaMethodImmunohistochemical technique was used to determine the expression of NS and TBX2 in 58 case of papillary thyroid carcinoma tissue, in 25 cases of thyroid adenoma and in 20 case of normal thyroid tissue around thyroid carcinomaResults1. NS proteins in 58 cases of thyroid papillary carcinoma (PTC) organization,34 cases were positive, rate of expression 58.62%,30 cases with lymph node metastasis in 23 cases, rate of positive for the expression 76.67%,and 28 cases without lymph node metastasis rate is 46.43%, expressed in 25 patients NS benign thyroid tumor tissues express positive rate 16.00%,NS proteins in malignant tissue of the genetic protein expression rate is significantly higher than in benign adenomas organizations and cancer in normal tissue adjacent to expression rate, the difference is remarkable,Abnormal expression of NS is unrelated,with the age and gender (P>0.05),which is related with infiltrating degree,pathological staging,lymph node metastasis relevant (P<0.05)2. TBX2 proteins have 51 cases positive in 58 cases of thyroid papillary carcinoma tissues, positive expression rate 87.93%,14 cases express positive in 25 cases of thyroid gland tumor tissue,11 cases express negative,ration of expression expression is 56.00%, ration of positive expression is 20.00% in normal tissue adjacent to carcinoma,negative expressing for 20 cases, positive expressing for 4 case,the positive expression ration of TBX2 protein in thyroid papillary carcinoma tissues is higher than in normal thyroid tissue,the difference is remarkable (P<0.05),and,the positive expression ration of TBX2 protein in thyroid papillary carcinoma tissues is higher than in normal thyroid tissue,the difference is remarkable (P< 0.05), thyroid papillary carcinoma tissues of high,medium differentiation are 39 cases in 58 cases, Statistics test results show that:7 cases express negative,23 cases express weak positive or positive,9 cases express strong positive, positive expression rate for 82.05%,in poorly differentiated thyroid papillary carcinoma organization for 19 cases,1 case express negative,7 cases express weak positive or positive,11 cases express for strong positive, positive expression ration is 94.74%, Statistics test results show that:the expression of TBX2 protein in the papillary thyroid cancer of high, medium tissue is lower in poorly differentiated thyroid papillary carcinoma tissues,the difference is remarkable(P<0.05).3. Common with TBX2 NS positive expression is 23 cases in 58 cases of thyroid papillary carcinoma,4 cases show lymph node metastasis,the ration is 6.80%,35 cases express incomplete and positive express or simultaneously, occupied for 71.42%, Statistics test results show:x2=16.211 P<0.001,the difference is remarkable(P<0.05).4. The relevance of the protein expression TBX2 and NS expression in thyroid papillary carcinoma:34 cases in 58 cases all expressed (+),13 cases all expressed (-),statistics test results show:P<0.001,both are positive correlation, Correlation coefficient r=0.613.Conclusions1. The NS and TBX2 were overexpression in papillary thyroid carcinoma,which clewed differentially expressing of NS and TBX2 may be involved in thyroid papillary carcinoma events. NS and TBX2 while expression are closely related with lymph node metastasis in papillary thyroid carcinoma,which clewed papillary thyroid cancer cell surface there might be exist secreted oneself ring.2. The NS protein and TBX2 protein were highly expressed in papillary thyroid carcinoma, which was positively correlated,which clewed both of them may play a synergistic action for occurrence of thyroid cancer, may promoted thyroid papillary carcinoma occurs through the cell hyperplasia level of regulating.3. The puncture of thyroid nodule with sample NS,TBX2 protein joint test, may play an important role for the early diagnosis and treatment of thyroid cancer... |
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