| Objective: To explore methods of establishment and evaluation of deficiency-cold model in Rats, and to abserve the effect of monkshood, with heat properity acroding to the theory of TCM, on energy metabolism and on relevant indicators in deficiency-cold model in rats. To analyse the tendency and speciality of these effects, then find the signs of cold and heat, and reveal the inherent relationship to cold and heat properity of TCM. To provide a useful guidance for clinical medicine.Methods: In order to induce deficiency-cold model in rats, decoction of four Chinese medicinal herbs with cold property, gypsum, radix gentianae, golden cypress and rhizome anemarrhenae decoction were administered intragastrically to rats for 14 days. After the model was successfully produced, decoction of mankshood was given for 7 days for treatment. The effects on temperature and cold and heat preference in deficiency-cold model rats after drug administration were observed. Collecting the food, water, urine and dung in quantity of rats for 24 hours with metabolic cage .The EI, DE, FE and ME were detected by Oxygen Heat Calculation. T3, T4, TSH, TRH in blood serum were analyzed using Enzyme-linked immunosorbent assay. Fasting blood sugar, hepatic glycogen and LD levels were measured. The activities of hepatic ATPase, LDH and SDH in liver were analyzed using measuring kit respectively. The EC and the content of ATP in liver were dected using HPLC.Results: Rats in model group looked coloured and dirty, with obvious diarrhea. Body weight, autonomous activities and toe temperature reduced significantly. After the treatment with Mankshood, plasma pyruvate, T3 levels in blood serum decreased. T4 level in blood serum and hepatic LD level increased, and the activity of ATPase, LDH and EC in liver increased. The declining trend was shown in those indicators, such as fasting blood sugar, weight, water and urine in quantity. The rising trend was shown in those indicators, such as the activity of SDH, the content of ATP. Conclusions: Using gypsum, radix gentianae, golden cypress and rhizome anemarrhenae can successfully induce deficiency-cold model in rats. Mankshood can enhance energy metabolism in deficiency-cold model rats and improve the rat deficiency-cold state, the pharmacology mechanism may be related to increase the hepatic energy metabolism and thyroid hormone. Effective appraisal indicators of the cold and heat property of TCM include weight, temperature, cold and heat preference, thyroid hormone, EC, the activity of hepatic ATPase, LDH and SDH. |
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