Pharmaceutical And Pharmacokinetic Research On Radix Scutellare And Radix Bupleuri In Compatibility

Objective: To analyze effective substance of Radix Scutellare and Radix Bupleuri in compatibility enhancing and changing the treatment that was proved to enhance drug effect by tcm clinical practice and pharmacological effect, in order to provide theoretical basis of modern medicine science for reasonable combination of tcm doctors prescribing medicine.Methods: Through consulting the related literature and learning about the research survey of Scutellaria and Bupleurum to lay a foundation for the experiments; high performance liquid chromatographic was used to determine the contents of baicalin and saikosaponin in sample solution;make reference to the clinical use of the drug, take extracting solvent, dosage, soaking time and extracting times by the four factors and three levels orthogonal test L9(3~4),and choose the optimum extraction process of tested plants. And baicalin contents in the plasma were measured by high performance liquid chromatography; the parameters of pharmacokinetics were calculated by DAS2.0 software.Results: The influence of extraction solvent on extraction ratio of medical components from Radix Scutellare and Radix Bupleuri in compatibility was studied in pharmacy experiment, and with baicalin as indicators, the alcohol extraction process of the drug pair was better than the extraction. The experimental results showed that combined decoction raised the solubilization capacity obviously, and the rationality and validity of the Chinese medicine drug pair are verified in the view of chemical composition. The best extracting condition was with 8times amount of 70% ethanol to operate heating circumfluence, for 3 times and 1h per time. The results showed that different compatibilities of Radix Bupleuri and Radix Scutellariae had small difference, and different compatibilities also had little effect on the content of baicalin, but the peak area of one absorption peak in front of baicalin increased evidently, so combined Radix Scutellare and Radix Bupleuri could enhance the extraction rate of the other constituents except baicalin;In pharmacokinetics study, after adopting 1:1drug -pair of Radix Bupleuri and Radix Scutellariae,baicalin was absorbed more rapidly in vivo than Radix Scutellariae,and the absorption peak of baicalin was moved forward by 0.34h, so the 1:1 drug -pair of Radix Bupleuri and Radix Scutellariae improved the rate to absorpt baicalin, making medicine efficacy of baicalin time advance, in addition, the secondry absorption peak of baicalin extended the retention time in rat in vivo after adopting the coupled drug dispensing of Scutellare– Bupleuri, and the absorption trend of baicalin in vivo of the 1:2 coupled drug dispensing of Scutellare– Bupleuri was similar with the 1:1 coupled drug dispensing, so the compatibility of Scutellare– Bupleuri not only promoted the absorption of baicalin in rat in vivo but also prolonged medicine efficacy time of it.Conclusion: By researching pharmaceutical and pharmacokinetic research on drug pair of Scutellaria and Bupleurum based on system science, determine the optimal extraction method,the best extraction technology and compatibility way, and the study on the coupled drug of Scutellaria and Bupleurum can be scientifically and perfectly ,thereby, we can establish solid foundation for the research and development of the Chinese medicine drug pair.