| 【Background】Gastric cancer has higher disease rate in alimentary system,especially in Asian countries, such as Japan, Korea, and China.Gastric cancer has become one of the diseases which does seriously harm to people's physical and mental health.At present,for gastric cancer treatment basically there are surgery, radiotherapy and chemotherapy and biological treatment etc. With various methods exist for the pros and cons, the therapeutic effect of gastric cancer has not yet reached the expected. Because the clinical manifestations are not specific in early stage of gastric cancer, little patients go to see the doctor. When patients with gastric cancer were found clinically, they were already in advanced or late stage, and they missed the best chance of surgery, which caused low rate of surgery. As the same time, patients have large suffering by surgery.Radiotherapy and chemotherapy drugs are double-edged swords, for their pharmacological properties, the cytotoxicity of most drugs have low specificity, when the drugs kill tumor cells, the normal cells are damaged at the same time, and cause some side effects to patients. Therefore, there is necessity to find a stronger safety and higher specificity drug to restrain cancers.Research results show that serum cholesterol levels and gastric cancer have associativity in pathologically classified, differentiation degree and so on.Lots of cholesterol were needed for the growth of tumor, and the metabolism of low density lipoprotein were very high, so patients with malignant tumors often had hypoalbuminemia. The level of serum cholesterol was lower obviously than normal because of the requirement for cholesterol in cells mitosis of gastric cancer, cancer cells absorbed a mass of cholesterol form serum, leading to metabolism disorders of cholesterol in patients, thus hypocholesterolemia was showed. Based on the theory above, we could use lipid-lowering drugs to reduce serum cholesterol, thereby it could affect cells mitosis of gastric cancer and inhibit the growth of cancer cells.In our study, we used lovastatin to reduce serum cholesterol, observe the effect of lovastatin to the size, weight and cells of xenografted gastric cancer and discusses the mechanism preliminary, so new theory basis and treatments could be provided for clinical.【Objective】To investigate the effect of lovastatin on the gastric cancer line SGC7901, to test the inhibitory effect of lovastatin on the growth of xenografted gastric cancer line SGC7901 in nude mice according to the request of guidelines for pre-clinical drugs, and to explore the related mechanism.【Methods】1. Human gastric cancer SGC7901 cell line was inoculated into armpit axilla skin of BALB/c nude mice to establish the model of xenografted gastric cancer line sgc7901 in nude mice. We used the model to asses anti-tumor activity of lovastatin.2. BALB/c nude mice were divided into 3 groups randomly: lovastatin group,30 mg/kg dose of Lovastatin was given continuously once a day for 3 weeks; 5-FU group, 45mg/kg dose of 5-FU was given continuously by intraperitoneal injection once a week for 3 weeks; control group, the same volume CMCNa was given once a day. The size and RTV of the tumor were observed.3. Killing the mice, stripping the tumor, weighing the tumor and calculating T/C(%) and the inhibition rate of tumor(%). 4. At last, the pathological morph and biological behaviour of the tumor were observed by optical microscope.【Results】1. Tumor were touched in each group 3 to 5 days after SGC7901 cell line was inoculated into BALB/c nude mice. 2. In 5-Fu group, the size of tumor were measured the relative tumor size,RTV was smaller than control group, the difference was significant (P<0.05 or 0.01), and T/C was 49.87%, the inhibition rate of tumor was 58.65% for xenografted gastric cancer line SGC7901 in nude mice.3. In lov group, the size of tumor were the relative tumor size,RTV was smaller than control group, the difference was significant (P<0.01), and T/C was 65.96%, the inhibition rate of tumor was 39.42% for xenografted gastric cancer line SGC7901 in nude mice.4.In light microscope, the negative group,8 lovastatin group, six 5-Fu group of tumor necrosis is obvious, two lovastatin group, four 5-Fu group of tumors did not change significantly avascular necrosis.The number of microvessels between tumor was no significant difference.【Conclusion】1,Lovastatin could inhibit the growth of xenografted gastric cancer line SGC7901 in nude mice.2,The congclusion of the research will supply new theray and evidence in gastric treatment... |
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