Effect Of The Interlukin-17 On The Proleferation And The Cell Cycle Of The Hepatic Stellate Cells

[Objective] To investigate the effect of the interlukin-17 on the proliferation and the cell cycle of the hepatic stellate cells (HSC)[Methods] The HSC were treated with IL-17(200,400,600,800,1000μg/L)for 24 hours,48 hours and 72 hours, respectively. The blank control group were the HSC treated without IL-17. the HSC were cultured in a RPMI 1640 medium in CO2 gas incubator for 24,48,72 hours,traced the growth curve using the cell counts of the three days. The cells were seeded in culture plates of 96 wells and monoplast suspension was harvested after trypsinization, then after 24 hours in CO2 gas incubator, The HSC were treated with IL-17(0,200,400,600,800,1000μg/L)for 24 hours,48 hours and 72 hours, respectively, with five replications per group,then the absorbance (A) was measured by the MTT assay after the MTT was added. Cells were seeded in culture plates of 6 wells with RPMI 1640 medium, with three replications per group, under the synchronized disposition, The cells were then treated with IL-17(0,200,400,600, 800,1000μg/L)for 48 hours and 72 hours, respectively, and cells were then collected and dyed by PI for determination of cell cycle by using flow cytometry.[Results]1. Compared with the control group, after stimulating the HSC by different concentration of the IL-17,the quantities of the cells did not increased after 24 hours. The quantities of the cells began to increase after 48 hours, the quantities of the cells increased obviously after 72 hours.2. Compared with the control group, different concentration of the IL-17 had no significant stimulation effect on the HSC after 24 hours (all of P>0.05), the IL-17(400,600,800μg/L) had stimulation effect on the HSC after 48 hours. The IL-17(400,600,800μg/L) had most significant stimulation effect on the HSC after 72 hours, the IL-17(800μg/L)had most significant stimulation effect on the HSC after 72 hours(P<0.01).however, the IL-17(1000μg/L) could inhibit the proliferation of the HSC, the inhibition effect was not significant after 24 hours, the inhibition effect began to appear after 48 hours, the inhibition effect was most significant after 72 hours.3. Compared with the control group, different concentration of the IL-17 had no significant effect on the cell cycle of the HSC after 24 hours.The percentage of cells at s phase began to increase after the stimulation of the IL-17(400,600,800μg/L) for 48 hours, and the percentage of cells at G0/G1 phase began to decrease at the same time. The percentage of cells at s phase began to increase significantly after the stimulation of the IL-17(400,600,800μg/L) for 72 hours, and the percentage of cells at G0/G1 phase began to decrease significantly at the same time, and the cell cycle give priority to the s phase, proliferation index increased with the concentration of the IL-17 in the given time span, proliferation index of the HSC was highest by the stimulation of the IL-17(800μg/L),and the proliferation index of the HSC was highest by the stimulation of the IL-17(800μg/L) after 72 hours. The IL-17(1000μg/L) could inhibit the proliferation of the HSC, its inhibition effect was not obvious after 24 hours and was obvious after 48 hours, its inhibition effect was most obviously after 72 hours. It could block the cells at G1 phase(P<0.01). All of the results had statistical significance.[Conclusions]1. The IL-17 could stimulate the proliferation of the HSC, which were associated with the dose of the IL-17 and the incubation time, the IL-17(800μg/L)had most significant stimulation effect on the HSC after 72 hours(P<0.01).2. The IL-17 could change the distribution of the cells at different phases of the cell cycle, it could promote cells from the G0/G1 phase to S phase.