| Objective:To study the effects of soy isoflavones on metabolic components of rats with metabolic syndrome induced by dietary and to explore the mechanism how SI prevent and treat MS from the viewpoint of metabolism of adipose cytokine.Methods:Experimental male SD rats model with metabolic syndrome were replicated by feeding high-fat and high-salt and high-glucose diet for 20 weeks.37 model rats with metabolic syndrome were established successfully and were randomly divided into 4 groups:model group,soy isoflavones high-dosegroup,soy isoflavones low-dose group and Metformin Hydrochloride group.These groups were separately given with the appropriate drugs for 4 weeks.To evaluate model and drug effectiveness by measuring weigh,blood pressure,fasting blood glucose,serum insulin,lipids and other indicators,insulin sensitivity index(HOMA-IR)continuely. After drug therapy, we observed the change of FFA level by using colorimetric method and measured relative quantitative expression of liver PPARamRNA by using RT-PCR method.Results:Compared with normal group, the weights and the blood pressures of experimental group rats which were given with high-fat and high-salt and high-glucose diet for 8 weeks had increased obviously(P<0.01). In the end of the 20 weeks,the index of serum insulin, TG, LDL in experimental group rats had heightened significantly (P<0.01) as well as TC increased (P<0.05), but HDL decreased without statistical significance (P>0.05).Compared with the vacuity group, experimental group rats had increased significantly in the terms of the weight, abdominal circumference, blood pressure, fasting blood glucose, fasting insulin, HOMA-IR, TG, TC and LDL whereas HDL level had decreased significantly (P<0.01).After drug treatment for four weeks, compared with the model group, the weight.Lee's index,visceral fat weight,the ratio of visceral fat and body weight in SI high-dose group and the metformin group had decreased significantly (P<0.01).There was no significant difference between two groups(P>0.05).The blood pressure in SI high-dose group had decreased significantly (P<0.05).The blood glucose,the serum insulin level and the insulin resistance index (HOMA-IR)in metformin group(P<0.01) and SI high-dose group(P<0.05) had decreased significantly. There was no significant difference between SI high-dose group and the metformin group (P>0.05). Lee's index in SI low-dose group had decreased significantly (P<0.01). Length of rats in each group had no significantly difference (P>0.05). The level of TG, TC and LDL-C in SI high-dose group rats had significantly decreased (P<0.01) and the level of TC in SI low-dose group had decreased significantly (P<0.05). There were no significantly difference between metformin group and model group in terms of lipid indexes (P>0.05).The level of FFA in the model group had increased significantly(P<0.01). After drug treatment for four weeks,compared with model group, the level of FFA in SI high-dose group and SI low-dose group and metformin group had decreased significantly(P<0.01),but the levels were still higher than control group(P<0.01).There were no statistic difference between SI high-dose group and SI low-dose group and metformin group(P>0.05)The relative quantitative expression of liver PPARamRNA in model group had decreased significantly(P<0.01).Compared with model group,the gene expression in SI high-dose group and metformin group had increased significantly(P<0.01).There were no difference between SI high-dose group and metformin group(P>0.05).Conclusion:1.Experimental male SD rats model with metabolic syndrome were replicated successfully by feeding high-fat and high-salt and high-glucose diet. This animal model had the basic features of MS.2.Soy isoflavones could reduce weight,lower blood pressure,regulate lipids and improve insulin resistance. Soy isoflavones had an important effect on preventing and treating MS.3. Soy isoflavones could increase the gene expression of liver PPARamRNA so that it could decrease the level of serum FFA in model animals. |
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