Serum Phospholipid Fatty Acid And Nuclear Factor KappaB In Patients With Metabolic Syndrome

Objective:To investigate the relationship between nuclear factor kappaB(NF-κB) and the serum phospholipid fatty acid in patients with metabolic syndrome(MS).Methods:60cases of MS patients and60normal control subjects (control group) were involved as the object of study.The level of height,weight,systolic pressure(SBP),diastolic pressure (DBP),waist circumference(WC), hip circumference(HC), fasting plasma glucose(FPG),2h plasma glucose during oral glucose tolerance test(2hPG),fasting insulin(FINS), hemoglobin(HbA1c),uric acid(UA),total cholesterol (TC),triglyceride (TG),high density lipoprotein(HDL-C),low density lipoprotein (LDL-C) were measured. Body mass inde x(BMI),waist hip ratio(WHR),index of insulin resistance (HOMA-IR) were calculated. Serum phospholipid fatty acid profile was analyzed with gaschromatography-mass spectrometer.ELISA method was used to measure the express of NF-κB in serum.T test was used to comparative analysis between the two groups,and Pearson correlation analysis was used to screen the influence factors of NF-κB.Results:The expression level of NF-κB in MS group was significantly higher than that in control group(P<0.01).And the level of SBP,DBP,weight,BMI,WC,WHR,HbA1c,FPQ2hPQFINS, HOMA-IR,UA,TG,LDL-C in MS group was significantly higher than those in control group (P<0.01-0.05).The percentage of saturated fatty acid (SFA) were higher than those in control group, but the percentage of polyunsaturated fatty acid(PUFA) were lower than those in control group.The percentage of monounsaturated fatty acids (MUFA) between the two groups didn’t have the statistical difference.The Pearson’s correlation analysis showed that the level of NF-κB was positively correlated with.weight,BMI,WC,HbA1c,FPG,2hPG,LDL-C,HOMA-IR(P<0.01-0.05),and.was.nega tivey correlated with HDL-C.Most importantly,the expression level of NF-κB was positively correlated with SFA(r=0.42,P=0.003),but negatively correlated with n-6PUFA and n-3PUFA(r=-0.33,-0.19,P=0.00,0.03,respectively).Conclusion:This.study.suggests.that.MS.patients.have.elevated.NF-κB.levels,Insulin.resistance, obesity,hyperglycemia,high lever of LDL-C and low lever of HDL-C can lead to increased activation of the NF-κB,and promote the development of MS.MS patients have. increased. proportion. of. SFA, educed.proportion.of.PUFA, and.these.changes. in serum.phospholipid.fatty.acid.compositions.are.corelated.with.the.increased.expression. of.NF-kB,suggesting that the increased SFA and reduced PUFA in the dietary pattern maybe caused or aggravated the inflammatory state of MS through the overexpression of the NF-kB pathway.