The Association Of Leptin Receptor Gene Polymorphisms With TCM Syndrome Characteristic In Hypertension Patients With Impaired Glucose Regulation

Purpose:The aim of this study is to explore the association of leptin receptor gene polymorphism with TCM syndrome characteristics in hypertension patients with impaired glucose regulation by using the molecular biology techniques, then we can presume the genetic essential characteristics of TCM syndrome in hypertension with impaired glucose regulation.Methods:All of the observations come from Jiangsu TCM hospital Cardiovascular out-patient and in-patient Department. The total number of the observations is 61, including 11 liver yang hyperactivities,23 yin deficiency yang hyperactivities,9 yin yang concurrent deficiency,18 phlegmatic hygrosis. Vital signs, biochemical indicators and OGTT results were observed. Take the patients fasting venous blood 2 ml to observe the leptin receptor gene Lys109Arg polymorphisms by PCR. Statistical analysis was done with SPSS software 17.0.Results:(1) Leptin receptor loci Lys109Arg genotype were divided into AA, AG, GG, allele A, G;(2) Comparing the clinical features of Lys109Arg locus genotypes, the results showed that different genotypes were significantly different in LDL-C (low-density lipoprotein cholesterol), FINS (fasting insulin), Homa-IR (insulin Resistance index) (P<0.05); GG genotype FINS, LDL-C was significantly higher than that of AG-type (P<0.05); GG genotype FINS, Homa-IR was significantly higher than the AA type (P<0.05); G allele (AG +GG genotypes) Homa-IR was significantly higher than non-G allele (AA genotype); non-A allele (GG genotype) FINS significantly higher than with A allele (AA+AG genotype);(3) The ages of yin yang concurrent deficiency in hypertension patients were significantly higher than others(P<0.01), and which of phlegm dampness were lower than the ones of yin deficiency yang hyperactivities(P<0.05).The patients of phlegm dampness were higher than the others in BMI, TC, LDL-C, FINS, and also higher in Homa-IR comparing with both liver yang hyperactivities and yin yang concurrent deficiency.(4) The distribution of LepR Lys109Arg genotypes in TCM syndromes of hypertension was different. AA genotype frequency in the syndrome of liver yang hyperactivity was higher than the one in yin deficiency yang hyperactivity(P<0.05); but as AG genotype, just be opposite in the two syndromes(P<0.05). Conclusion:The results show that, LepR gene Lys1O9Arg SNP polymorphism may have some relationship with the TCM syndrome in hypertension patients with impaired glucose regulation; Lys1O9Arg Mutation can induce the abnormality of glucose and lipid metabolism, as We have a deeper molecular understanding of the level of impaired glucose regulation and know hypertension essential characteristics of TCM.