| Background and ObjectiveA serious of pathophysiological changes on ventricular structure, ventricular size and cardiac function will occur after acute myocardial infarction. Myocardial interstitial fibrosis will form gradually in infarcted area, myocardium compensatory hypertrophy will happen in non-infarcted area, the wall of the ventricular will thicken gradually, the myocardium in infracted area will swell out, the ventricle will progressively expand, the systolic and diastolic function will decreased sharply. And all these changes will result in ventricular remodeling. Mocardial blood supply will improved only at a certain extent by current treatment on myocardium infarction, and there is no better treatment for the necrotic myocardium. It was found that the cardiac function was improved significantly and the ventricular remodeling was prevented by bone marrow stem cell. And transplantation of bone marrow stem cell on treatment myocardial infarction has become a hot spot in modern cardiovascular field now. In this study, We setup a acute myocardial infarction model to observe the effect of allogenenic bone marrow mononuclear cells transplantation on the concent of type I collagen, gene expression of angiotensionⅡ(AngⅡ) and ostepontin (OPN) after acute myocardial infarction.Explore the possible mechanism of ventricular remodeling after AMI so as to provide theoretic basis for clinic application of bone marrow stem cells transplantation to cure ischemic heart disease.Materials and methods1.48 healthy SD rats were randomly divided into 3 groups:control group (n=16) AMI group (n=16) and and transplantation group(n=16). In control group, the rats' chests were opened without coronary artery ligation, while in AMI group and transplantation group, the rats' left anterior descending branch of coronary arteries were ligatured to establish the AMI models. Then around the infarcted left ventricular area of the rats in transplantation group were injected with BM-MNCs suspension beneath the epicardium, and those of AMI group were injected with LG-DMEM culture solution.2. After 4 weeks of the operation, the weight, cardiac weight index, left ventricular weight index, content of typeⅠcollagen, expression of OPN mRNA (osteopontin gene) in the non-infracted myocardium of left ventricle,and the AngⅡlevel in the non-infracted myocardium of left ventricle were measured.ResultsAfter 4 weeks of the operation, transplantation group and AMI group were significantly decreased while the content of typeⅠcollagen, AngⅡand the expression of OPN mRNA in the non-infarcted myocardium of left ventricle, comparing with control group (P<0.05 for all). Comparing with AMI group,the level of the heart weight, left ventricular weight, heart weight/body weight, left ventricular weight/body weight, content of typeⅠcollagen, AngⅡ, expression of OPN mRNA in the non-infarcted myocardium of left ventricle of transplantation group were significantly decreased(P<0.05 for all).ConclusionsTransplantation of allogenic bone marrow mononuclear cells could prevent ventricular remodeling, improve cardiac function after AMI, possibly by reducing the synthesis of typeⅠcollagen in the non-infarcted myocardium, depressing the expression of AngⅡand OPN gene. However, the exact mechanism needs further studies for the allogeneic bone marrow mononuclear cell transplantation to prevent ventricular remodeling after acute myocardial infarction. |
PDF Full Text Request