| Objective: To compare acute myocardial infarction transplantation of autologous bone marrow mononuclear cells (BM-MNCs) and mesenchymal stem cells (MCSs) for the improvement of cardiac structure and function.Method: 1.Taken under sterile conditions on dogs after iliac crest bone marrow of about 20ml, by density gradient centrifugation of bone marrow mononuclear cells, and a "priority" selection method further to obtain adherent bone marrow mesenchymal stem cells cultured in vitro expansion increase, up to about 108. 2.24 dogs were randomly divided into three groups: BM-MNCs transplanted group (8), MCSs transplantation group (8), control group (8). 3.By way of coronary artery balloon occlusion in dogs with acute myocardial infarction. 4.After acute myocardial infarction model using the OTW balloon occlusion prior to the blood, transplant group were injected mesenchymal stem cells and mononuclear cells into the equivalent control group PBS buffer. 5.Respectively, before and after tooling around underwent echocardiography, cardiac cycle of three consecutive measurements taken to detect left ventricular end diastolic diameter (LVEDd) and end systolic diameter (LVEDs), measured and calculated left ventricular ejection fraction (LVEF). 6.Respectively, and after tooling around the time after the review using the pigtail catheter into the left ventricular cavity, with the simultaneous determination of ECG left ventricular systolic pressure and left ventricular end diastolic pressure, left ventricular blood pressure within.Results:1.24 canines were completed coronary angiography and balloon occlusion, 28 survived 20 days, two LAD in coronary pressure during balloon-induced ventricular fibrillation was killed, while two made at the AMI model within 24h after the death of massive bleeding puncture after death. 2.Preoperative BM-MNCs transplantation group, MSCs transplantation group and control group LVEF was (72.50±3.88%; 73.33±1.96%; 73.00±2.97%), no significant differences among the three groups (P = 0.923) . After 4 weeks, three VLEF were (60.00±4.33%; 61.33±3.01%; 48.33±1.96%), differences among the three groups was statistically significant (P = 0.000). MSCs transplantation group, LVEF was slightly higher than BM-MNCs transplanted group, but the difference was not statistically significant (P = 0.452). 3.Among the three groups before surgery LVEDd and LVEDs There were no differences (P = 0.779, P = 0.990), after 4 weeks compared with that in different degrees of increase in the control group and LVEDs expansion LVEDd transplantation group compared with the two large the difference was statistically significant. BM-MNCs compared with the control group, LVEDd, LVEDs were significantly different (P = 0.006,0.001) MSC group compared with control group, LVEDd, LVEDs were significantly different (P = 0.005, P = 006), but BM-MNC group compared with the MSC, LVEDd, LVEDs no significant difference (P = 0.895, P = 0.452). 4.the preoperative invasive measurement of left ventricular outflow trace pressure BM-MNCs transplatation group and MSCs transplatation group and control group .LVSP,LVDP among lthe three groups showed no significant difference (P=0.215,P=0.245). After 4 weeks, BM-MNC group, and MSC, respectively, compared with the control group, LVSP was no significant difference (P = 0.852, P = 0.852). After 4 weeks, BM-MNCs transplantation group, MSCs transplantation group compared with the control group, respectively, were significantly different (P = 0.001, P = 0.0000).Conclusion:1. Interventional coronary autologous stem cell transplantation for acute myocardial infarction in the method is feasible.2.Acute myocardial infarction after vascular reperfusion for BM-MNCs and MSCs in the coronary graft, the infarction systolic function improvement. And to reduce left ventricular remodeling process, reducing ventricular expansion. But in the short term after myocardial infarction (4 weeks) between the two treatment no significant difference. |
PDF Full Text Request