| Backgroud and purposeOvarian cancer is one of the most common malignant tumor of gynecology, the mortality rate ranks first in gynecological malignancies. In recent years, with the average life expectancy increasing, the rapid development of science and technology, and the continuous development of ovarian cancer diagnosis, the detection rate of ovarian cancer increased year by year, but there are not obvious symptoms of early ovarian cancer and signs, there are not high specificity and sensitivity tumor markers and other diagnostic methods, so ovarian cancer is often diagnosised when it has been terminal, although the treatment has been significantly improved, the mortality rate of ovarian cancer is still high and increasing year by year. So how to found and diagnose ovarian cancer when it was early stage and its correct and effective treatment became the focus of attention. Proto-oncogene Pim-1 is a member of the Pim gene family (now have been discovery:Pim-1, Pim-2, Pim-3), was first found in the Moloney murine leukemia virus (Moloney murine leukemia virus, MoMuLV) induced The T-cell lymphoma[1], Pim-1 kinase which is encoded by Pim-1 gene is a serine/ threonine kinase (serine/threonine kinase), studies confirmed that the overexpression of this kinase is related to the occurrence and development of many tumors, such as leukemia, lymphoma, prostate cancer[2] and gastric cancer[3] and so on. Proto-oncogene C-myc encodes a nuclear phosphoprotein, some research suggests that it was high expression in ovarian cancer[4].But there is no report about the study of the expression of Pim-1 gene and its protein in ovarian cancer tissue and the correlation of Pim-1 and C-myc in ovarian cancer at home and abroad. In this study, we will detect the expressions of Pim-1 and C-myc in epithelial ovarian cancer by using semi-quantitative RT-PCR method and immunohistochemical, and explore the relations among their and the occurrence and development of the epithelial ovarian cancer.Materials and Methods1 Experimental samples:Choose 70 cases of patients who saw a doctor at the First Affiliated Hospital of Zhengzhou University in October 2008-March 2010, by nature, they are divided into 3 groups:patients with epithelial ovarian cancer 40 cases, (malignant group), age 36-70 years, the median age:51.0 years, in which:serous adenocarcinoma 29 cases, mucinous cystadenocarcinoma 11 cases,Ⅰ-Ⅱstage 14 cases,Ⅲ-Ⅳstage 26 cases, lymph node metastasis 23 case, without lymph node metastasis 17 cases; Patients with epithelial ovarian benign tumor 20 cases (benign group), patients age 30-69 years, the median age:47.5 years; (patients with ovarian resection due to uterine fibroids) Control group of normal ovarian tissue 10 cases (normal group), aged 45-68 years, the median age:50.5 years, there is not significant difference among three groups's age (P> 0.05). All the selected cases don't have the history of other tumors, ware not radiotherapy and chemotherapy before surgery and were confirmed by pathology after surgery. Take all the fresh ovarian tissue removed in surgery of the selected patients, and divide it into two, one immediately stored in liquid nitrogen, used for RT-PCR; another fixed with 10% formaldehyde solution, paraffin pack buried, used for immunohistochemistry.2 Experimental Methods:Detect expressions of Pim-1, C-myc gene and protein in epithelial ovarian cancer tissue, benign epithelial ovarian tumor tissue and normal ovary tissue by using Reverse transcription-polymerase chain reaction (RT-PCR) semi-quantitative methods and immunohistochemistry.3 Statistical analysis:application SPSS 16.0 statistical software analysis:Statistical analysis of quantitative data applied Kruskal-Wallis test, of statistical analysis of qualitative data applied X2 test, correlation analysis using Spearman rank correlation.①=0.05 was inspection standards.Results1 The expressions of Pim-lmRNA and protein in epithelial ovarian cancer (1.241±0.203,72.5%) were significantly higher than their expressions in benign ovarian tissue (0.539±0.064,35.0%) and their expressions in normal ovary tissues (0.009±0.001, 10.0%), the difference was statistically significant (both P=0.000).2 The expressions of C-myc mRNA and protein in epithelial ovarian cancer (0.832±0.097,65.0%) was significantly higher than in benign ovarian tissue (0.447±0.038, 25.0%) and in normal ovary tissues (0.006±0.001,0), the difference was statistically significant (both P=0.000).3 The positive expressions rate of Pim-1 protein in serous and mucinous epithelial ovarian cancer were 69% and 81.8%, the difference was not statistically significant (P=0.677); The positive expression rate of Pim-1 protein in stage I-II and III-IV of epithelial ovarian cancer were 50% and 84.6%, the difference was statistically significant (P=0.049); The positive expression rate of Pim-1 protein with and without lymph node metastasis in epithelial ovarian cancer were 82.6% and 52.9%, the difference was statistically significant (P=0.043).4 The positive expression rates of C-myc protein in serous and mucous epithelial ovarian cancer were 58.6% and 81.8%, the differences was not statistically significant (P=0.316); The positive expression rates of C-myc protein in I-II stage and lymph node metastasis in epithelial ovarian cancer were 57.1% and 69.6%, comparing to the positive expression rates of C-myc protein in III-IV stage and no lymph node metastasis in epithelial ovarian cancer:69.2% and 58.8%, the differences were not statistically significant (P=0.445,P=0.481).5 In epithelial ovarian cancer tissue, Pim-1 and C-myc protein were all positive expression in 23 cases, while no expression in 8 cases, statistical analysis showed that there was significant correlation between them, and the correlation was positive (r=0.487, P=0.007). Conclusion1 Pim-1 and C-myc may be related to the occurrence of epithelial ovarian cancer.2 Pim-1 may be related to the invasion and metastasis of epithelial ovarian cancer, but there is not significant correlation between C-myc and the progression of ovarian epithelial cancer.3 Pim-1 and C-myc may play a synergistic role in the malignant transformation of epithelial ovarian cancer. |
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