Experimental Study Of Cepharanthine Hydrochloride On Treatment Of The Models Of Hyperlipidemic Fatty Liver In Mice

Background&Purpose:Hyperlipidemic fatty liver disease (HFLD) is one kind of non-alcoholic fatty liver disease. The simple steatosis may develop to steatohepatitis, liver fibrosis or cirrhosis. It is caused more attention by the high incidence. It is of great significance to develop the ideal drug to treat HFLD. To study and establish the animal model of hyperlipidemic fatty liver in mice and to observe the therapeutic effects of Cepharanthine Hydrochloride (CH) against the models induced by Dexamethasone (DEX) and high-fat diet in mice.Materials and methods:1 Three fatty liver animal models were produced by carbon tetrachloride (CTC), DEX and alcohol (ALC). The total cholesterol (TC), triglyceride (TG), alanine aminotransferase (ALT), aspartate amintransferase (AST) in the serum were measured.2 Hyperlipidemic fatty liver model (HFLM) was produced by injecting Dexamethasone Sodium Phosphate injection in the mice'abdomen and feeding mice high-fat diet at one time. The TC, TG, ALT, AST in the serum and the TC, TG in the liver were measured. Liver tissues were observed with optical microscope.3 The hyperlipidemic fatty liver mice models were prevented with different doses (low, middle and high) contrasting with Tiopronin. The TC, TG, ALT, AST in the serum and the TC, TG, superoxide dismutase (SOD), maeic dialdehyde (MDA), glutathion peroxidase (GSH-Px) in the liver were measured. Liver tissues were observed.Results:1 Compared with the normal control group, the activations of ALT and AST were increased significantly (p<0.01), TG and TC were no significant difference in the CTC group; The TC, TG, ALT and AST in the serum were increased significantly in DEX group (p<0.01); the activation of ALT was increased significantly, however, AST, TC and TG were no significant difference in the ALC group.2 Compared with the normal control group, the ALT, AST, TG and TC were all increased significantly, the level of TG and TC in liver homogenate and liver index were increased significantly, hepatic steatosis and inflammatory infiltration were observed by pathological examination in the HFLM group.3 With different dose of CH, hepatic steatosis was improved in dose-response and dose-dependent manner. Compared with the normal control group, the liver index, serum TG, TC, ALT and AST levels, and hepatic TG, TC, MDA level markedly increased (all P<0.01); and hepatic SOD and GSH-Px significantly decreased (both P<0.0l) in the model control group. Compared with the model control group, serum TG, TC, ALT and AST, and hepatic TG, TC, MDA markedly decreased(P<0.01 or P<0.05); and hepatic SOD and GSH-Px significantly increased(both P<0.01) in the high-dose Cepharanthine Hydrochloride treatment group.Conclusion:Mice HFLM were successfully established by injecting DEX plus high fat diet. The HFLD has metabolic disturbance of blood fat and hepatic injury simultaneously which is worth further comprehensive evaluation. CH has good therapeutic action on hyperlipidemic fatty liver disease. The possible mechanism is that CH can improve antioxidase activity and depress lipid peroxidation of fatty liver.