Clinical Observation Of Bushenzhuanggu Capsule In The Treatment Of KOA And The Effect On The Expression Of MMP-1, TIMP-1, ADAMTS-4

Objective :To observe the efficacy of Bushenzhuanggu capsule(BSZGC) for treatment of knee osteoarthritis(KOA) and its mechanism.To observe effects on knee osteoarthritis chondrocytes ADAMTS-4, MMP-1, TIMP-1 expression.Methods: In the experiment study ,40 New Zealand rabbits were randomly divided into 4 groups:Group A (the normal group),Group B (the model group),Group C(the treated group) and Group D(the control group).KOA models were created in Group B, C and D by Hulth's operation methods. The rabbits in Group D were treated with Zhuangguguanjie pill (ZGGJP) and BSZGC was used in Group C. 8 weeks later, to make CR of the knee, Then to kill the rabbits and get the femoral condyle and measured MMP-1, TIMP-1, ADAMTS-4 positive cells. Clinical research applications BSZGC,ZGGJP treatment of KOA of the 30 patients in the control and the overall clinical efficacy observed symptoms and signs improve the situation.Results : The experiment study showed the model group significantly articular cartilage degeneration, HE staining and light microscopy MMP-1, TIMP-1, and ADAMTS-4 expression showed that the treatment group and model group were significantly different(P <0.01), treatment group and control group of related indicators no significant differences detected (P> 0.05), Clinical studies have shown that application of BSZGC of KOA total efficiency and the control group were significantly different (P <0.05).Conclusions :MMP-1 and ADAMTS-4 expression may be too much of articular cartilage degeneration is one of the important reasons, BSZGC articular cartilage by increasing the expression of TIMP-1 and inhibition of MMP-1 and ADAMTS-4 expression to effectively improve KOA in patients with clinical symptoms and restore knee function.