Relationship Between Serum PCT,Polymorphisms Of IL-10 Gene And Sepsis

Objective:sepsis is now common in the neonatal intensie care unit is one of the most serious complications. Infection causes a sepsis and merge in critical patients MODS is the main cause of death. Global sepsis rate was 28% or so, but different age 10 different:children, aduLt 38%, elderly is 85%. Our current lack of detailed clinical epidemiological material, but according to calcuLation, each year couLd have 300 million patients sepsis occurred [2]. The aim of this study was to in order to understand the septic patients serum, discuss basis PCT level with sepsis PCT diagnosis systemic inflammatory response syndrome and relationship of judgement and prognosis of the threshold value and corrclation; Research IL - 10 gene single-nucleotide polymorphisms (SNPS) - 1082G/A,-592C/A site gene frequency distribution and sepsis susceptibility relationship. Reveal IL - 10 genes and sepsis relationship, as its pathogenesis and prevention study provided scientific basis for gene polymorphism will help for us to deeply analyzes the pathogenesis of sepsis, and explore the nature of disease phenotype diversity produce more individualized, development of clinical treatment measures and open up a new way.Methodsusing the method controlled study cases, collection, CPT (70 cases) and normal control group (30 cases), according to standard into septic group, systemic inflammation response syndrome group and control group, morning hollow take peripheral blood, serum, determination of calcitonin original (PCT), C-c-reactive protein,WBC and neutrophils percentage (N). XieChen (ESR) level, compared with a statistical significance. Between whether Take each research object peripheral leucocyte extraction genomic DNA, the amplification block mutation system polymerase chain reaction (PCR) and amplification and-extinction enzymes length polymorphism (PCR-RFLP) method, inspection IL-101082G/A genetic-592C/A site, genotype and alleles of the SNP, were two gene polymorphism. We concluded that the level of serum PCT and prognosis for the diagnosis and treatment of correlation between, provide the opinions; Research IL - 10 gene promoter area-1082G/A,- 592C/A gene polymorphism and sepsis and systemic response syndrome predisposition.Research resuLts 1. Health groups are lower than 0.5 PCT serum ng/ml, SIRS group increased serum PCT level has increased more than 52.5% in concentration, ng/ml of 0.5-2.0, sepsis group increased significantly,90% of patients increased more than the concentration of the 2.0-10.0 ng/ml; Septic patients PCT value significantly higher than SIRS group (P<0.05). and the resuLts proved the clinical significance of PCT detection. Patients will be based on PCT PCT> serum level 2 ng/ml into two groups were compared.> 2 PCT illustrated for diagnosing ng/ml sepsis standard, sensitivity (Pa), specific degrees (Pd) and accuracy (CA) were higher, respectively,85%,80% for 73.3% positie predictie were higher for allergic. Based on PCT, the diagnostic sepsis just 26.7% miss rate is also lower, the misdiagnosis rate of 15%. In the clinical application of sepsis can better for diagnosis and judgement.2. IL-10-1082G/A genotype (GG, GA, AA) in sepsis group, SIRS group and control group for the frequency of AA type 70% respectively,70% and 80%. GA type 23.3%, 25.0% compared with 16.7%. GG type 6.7%, cholesterol by 5.0% compared and 3.3%; IL-10-592 site AA, CA, CC 3 genotypes frequency in sepsis group, systemic inflammation response syndrome group and control group in distributed 70.0%, for AA type 50 per cent and 40 per cent, CA type 20% and 40%, and 33.3%, CC type 10%,10% and 26.7%. Allele (C. A) the frequency of the three groups respectively for 20%,30%.43.3% and 80%,70%,56.7%; Analysis found that, IL-10 americans in the genomes of SNP appear in the endless and same, the frequency with foreign crowd are obviously different 1082A compared the frequency,- far more than other popuLations; - 1082G/A genotype and A and G allele frequency in sepsis group, SIRS group and control group between three groups there are no significant difference (P> 0.05).592AA sepsis group - genotype frequency compared with controls, higher than those in the control group, and significant difference (P< 0.05), 592C allele frequency - compared with the control group, difference obviously lower than there was A significant significance (P< 0.05). Thus inference septic patients high frequency - 592AA genome genotype prompted IL - 10 low level secretion, although a 592C relative to a 592A for IL - 10 high expression type, but its expression allele frequency obviously lower than healthy crowd, the two jointly action to make septic patients in IL - 10 condition, to the low levels of the release of promoting inflammatory cells factor inhibition relatively weak, inflammatory cells factor with a large release, increases the risk of severe sepsis. So IL - 10592AA genotypes in with sepsis diseases. Single figure AC sepsis group, SIRS group and control group three significant differences between groups (P< 0.05), obviously lower than those of the control group, this single figure sepsis group. SIRS significant differences between groups (P< 0.05). Single size AA sepsis group, SIRS group and control group with significant difference (P< 0.05). but no significant difference between them (P> 0.05). AA single figure significantly lower than those of the control group of sepsis, confirmed the ATA single times with IL-10 of relevant. Produced reduce Single figure AC may also and the onset of sepsis risk relatedconclusionAs early PCT systemic inflammatory response syndrome and sepsis shortcut, sensitive and accurate means of detection. PCT> 2 ng/ml but as a diagnostic sepsis judgement point, has high sensitivity, specific degrees, accuracy and high level of positie predictie PCT, the prognosis of unfavorable to sepsis. IL - 10 with sepsis susceptibility gene polymorphism, IL - 10 - related 592AA genotype and the occurrence of sepsis related to development, a single figure AC type the probability of sepsis cases may be higher than other patients.