And HGF Regulaiton Synthesis Of Milk Fat And Milk Protein In Bovine Mammary Epithelial Cells

Cow is one of the economic animals in animal husbandry and aquaculture, andthe production and quality of the milk of the dairy cow are the main measurement offarming and economic standards. China is the famous dairy farming country, but theannual average yield of the cows below the world average, and the percentage of Milk Fat and the content of milk protein are relatively low, which have a serious impacton the consumer demand. The mammary gland synthesis and secretion milk, under the mediation of nerve-endocrine-immune network, and the changes of important function of the gene expression in mammary gland development and lactation processis have relationship to regulate mammary growth, development, differentiation, andmilk synthesis, secretion, transit and other important physiological process. Mammary epithelial cells is the implementation of the lactation. Mammary epithelial cells isregulated by the neuroendocrine system and the immune system. Under the combination of the effects of the neuroendocrine network systems to maintain normal lactation. Cytokines can directly act on the mammary epithelial cells, regulating the biosynthesis and secretion of milk protein, milk fat; cow mastitis is a serious risk factors, which have a direct impact on the cytokines type and the change of quantity. Understanding the impact of cytokines on lactation, studying the regulation of the synthesis ofmilk fat milk protein for lactating cells on the cellular level will provide a basis for artificial regulation of dairy quality.First, preparing the tissue explant, cloned and characterized the bovine mammary epithelial cells, mammary epithelial cells lactation model. Morphology, biology and function test experiments showed that mammary epithelial cells is a typical oval, the normal number of chromosome number and mammals; growth curves that meet the normal growth cycle in vitro; able to secrete β-casein, thus lactation as the mammary epithelial cells in vitro research model for studies on the regulation of late lactationplatform.Established bovine mammary epithelial cells lactation model, different concentrations of IGF-I (0,10,100,200ng/mL), and HGF (0,0.1,1,10ng/mL) were cultured for24h to stimulate real-time fluorescence quantitative transcription level of β-casein(CSN2), and acetyl coenzyme a carboxylase alpha (ACACA). Study IGF-I and HGFin the mammary epithelial cells in lactating.The results showed that compared with the control group, with the increase of IGF-I concentrations, CSN2mRNA levels in bovine mammary epithelial cells in thegradual increase in a concentration dependent manner; Similarly, ACACA mRNA expression in bovine mammary epithelial cells andCSN2has a consistent trend. HGF showed an opposite trend. With the increase of the concentration of HGF, CSN2andACACA in bovine mammary epithelial cells, the mRNA levels gradually decreased,dose-dependent manner. Therefore, this study conclude that: in a certain range of concentrations of IGF-I can significantly promote the synthesis of milk fat and milk protein, while HGF significantly inhibit the synthesis of milk fat and milk protein concentration-dependent manner.