Analysis Of DNA Barcoding On Partial Indigenous Duck Breeds

DNA Barcoding is a new generation of molecular marker for species (breeds) identification. It is based on a DNA segment on Chondria of reptile-- cytochrome oxidase subunit 1 gene to identify species. Nowdays, it is applied to Birds, fish species identification,but few report on domestic fowls.A pair of universal primers of DNA Barcoding was used to amplify domestic duck DNA genome and sequence. According the sequence of domestic duck COI gene, a set of nested primers was designed to amply duck COI sequence. The sequence difference was compared on three local breeds, Guangxi Xiaoma duck, Jingxi Dama duck and Gaoyou duck.30 blood sample were selected randomly from China waterfowl breed resource bank on every breed and COI was amplified and sequenced.The sequences were aligned by Clustal X software and the SNPs of the sequences were identified with DNASP software.The study revealed that there existed 12 polymorphic sites and 13 haplotypes in the sequence of CO I gene fragment. The haplotype diversity and average nucleotide diversity were 0.68%. and 2.15% respectively. Among 13 haplotypes, nine haplotypes can be assigned to special breed. MP tree was constructed based on sequence of 13 haplotypes and the MP tree coincide with the morphological characteristics. All sequences of 13 haplotypes was stored in Barcode and Genebank Database. The barcode of COI gene may be used for identifying duck breeds.