Tissue Culture Of Anthurium Andraeanum Cultivar Alabama

In this paper, in order to achieve a large number of Anthurium andraeanum cultivar Alabama plantlets in a short time, tissue culture system of A. andraeanum was established by optimizing processes. Hydroponics culture experiments of A. andraeanum plantlets were performed to replace cultivation with hole tray which would consume large amounts of manpower and material resources. Calli and plantlets of A. andraeanum were mutation-induced by (60)^Co radiation, and the range of semi-lethal dose was obtained. The main results outlined below:â‘´Establishment of tissue culture procedure of A. andraeanum cultivar Alabama:Condition of tissue culture of A. andraeanum cultivar Alabama was optimized at every stage and the operating procedure system was established. The procedure summarized as follows: i) Explants were sterilized first by immersing in ethanol for 20～30s and in solution of HgCl2 for 8min. ii) The sterilized explants were cultured on full-strength Murashige and Skoog (MS) media containing 1.0mg·L^-1 6-BA and 0.2 mg·L^-1 2,4-D to induce calli. iii) Calli were subcultured on MS medium containing 2.0mg·L^-1 6-BA and 0.2 mg·L^-1 2,4-D to induce adventitious buds. iv) The adventitious buds which growed well were subcultured on half-strength MS media containing 2.0mg·L^-1 IBA and 0.1 mg·L^-1 NAA to induce root. v) The rooted regenerated plantlets were transplanted after acclimatized.⑵Hydroponics culture experiment of A. andraeanum cultivar Alabama plantlets: Pre-treated with high concentrations plant growth regulators, cultured in solutions contained low concentration plant growth regulators and in different nutrient solutions were tested for hydroponics culture experiment of A. andraeanum cultivar Alabama plantlets. Results showed that the growth of plantlets pre-treated with 10mg·L^-1 IBA for 2 h, cultured in solution contained 0.5mg·L^-1 NAA and cultured in nutrient solutionâ…£was superior to other treatments respectively. Comparison of those three methods, results showed that solution of 0.5mg·L^-1 NAA was benefit to the root inducement of A. andraeanum plantlets and nutrient solutionâ…£could promote their growth. The formula of solutionâ…£is 40mg·L^-1 NH4NO₃,177 mg·L^-1 KNO₃,123.5mg·L^-1 MgSO₄·7H₂O,68mg·L^-1 KH2PO₄,7mg·L^-1 Na2Fe-EDTA,0.225mg·L^-1 MnSO₄·4H₂O,0.975mg·L^-1 H3BO₃,0.43mg·L^-1 ZnSO₄·7H₂O , 0.0375mg·L^-1 CuSO₄·5H₂O , 0.012mg·L^-1 (NH4)6Mo7O24·4H₂O.⑶Study of (60)^Co radiation on A. andraeanum cultivar Alabama under tissue culture conditions:This experiment used 5, 10, 20, 30, 40, 50 and 60Gy dose of (60)^CoγRay to radiate the calli and plantlets of A. andraeanum cultivar Alabama. Plant regeneration induced from the radiated calli later, and the result showed that (60)^Co radiation inhibited the growth of A. andraeanum. The radiated plantlets were much smaller than the control. The semi-lethal dose of callus was between 20～30Gy, and that of plantlet was between 30～40Gy. These semi-lethal doses can be targeted for further radiation experiments.