| This experiment was conducted to study the effects of the combination of fish meal and pig spary-dried blood cells in the diet for Crucian Carp on growth performance and approach to the effectiveness and feasibility of replacement of fishmeal by pig spary-dried blood cells which is achieved by optimal combination of feed ingredients. Six experimental diets were formulated isonitrogenously, isolipidicly,isoenergetic and isophosphorously. The total FM diet treatment without spray- dried blood cells was used as control, the other treatments were formulated with2.5%,5%,7.5%,10%and 11.5% spary-dried blood cells respectively,corresponding to the amount of fish meal were 13.8%, 10.7%, 7.8%, 4.8%, 1.8%, 0%. The levels of lipid and total phosphorus were balanced respectively by soybean oil and Ca(H2PO4)2 and the level of total nitrogen were balanced by cross combination of pig spary-dried blood cells and fishmeal . 270 fishes with initial mean weights of 18.40±1.07g were randomly assigned to six dietary treatments with three replicates and fifteen fishes with each pen. Growth and physical indicators, feed utilization, serum biochemical parameters, immune function, amino acid composition and apparent digestibility of amino acids and other indicators were analyzed after 83 days. The results showed that:1. Growth performance: Compared with the control,when the pig spary-dried blood cells added in the level of 7.5%, 10% and 11.5%, the specific growth rate, protein and fat deposition ratio were significantly increased, feed conversion ratio decreased significantly (P<0.05); the condition factor,viscerosomatic index,hepatopancrea index,kidney index and body composition showed no significant difference (P>0.05).2. Physiological function: the T-SOD, LSZ, Hb, AST, BUN, GLU, TCHO, TG, LDL-C was not significantly different (P>0.05). Compared with the control ,when spary-dried blood cells added more than 7.5%, GPT enzyme activity of liver was lower than the control and serum activity was higher than the control group, the difference was not significant (P>0.05).3. Amino acid composition: The correlation coefficient of fish body amino acids ,muscle amino acids and diets amino acids were0.90～0.93, 0.92～0.94 respectively, the correlation coefficient of liver amino acids and diets amino acids was over 0.99 . The correlation coefficient of serum, whole fish, muscle, spleen, kidney, brain, heart free amino acids and diets amino acids was over 0.90. The free amino acids'concent of the same organs and tissues of crucian carp was different, but the compositional patterns of free amino acids was relatively stable in different experimental diets.4. Apparent digestibility of amino acids: The apparent amino acid digestibility's differences among dietary treatments in different time periods after feeding was significant (P<0.05) .These results suggest that:①When the experimental diets were basically isonitrogenously, isolipidicly, isoenergetic and isophosphorously, with the pig spary-dried blood cells adding to the diet at 7.5% or less, more than 4.8% fish meal, soybean oil 1.9%, 2.7% monocalcium phosphate, the growth performance of fish was significantly improved; and also maintain a normal, stable physical health.②Under the experimental conditions, the fish whole fish, muscle amino acid composition and feed amino acids have a stronger correlation; different feed groups carp organs and tissues of the same pattern of free amino acids was no difference in relative stability.③The total amount of free amino acids in different organs and tissues is muscle> spleen> kidney> brain> heart; carp whole fish and muscle, heart and brain, kidneys and spleen composition of free amino acid pattern is very similar... |
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