Study On The Bioavailability Of Common Dietary Protein Source By Allogynogenetic Crucian Carp

The study contented three experiments, This present research was to evaluated the effect of different feed ingredients to the growth performance(weight gain, WG; specific growth rates, SGR)and the apparent digestibility of dry matter , crude protein , crude lipid and energy .This research was also to evaluated the effect of levels of cottonseed meal and gossypol to the growth performance(weight gain, WG; specific growth rates, SGR),physiological enginery(superoxide dismutase, SOD; lysozyme, LSZ; gliutamic-pyruvic transminase, GPT; glutamic oxaloacetic transaminase , GOT; hemoglobin, Hb), histology of liver and intestines on allogynogenetic crucian carp.And this research was also to investigate the condign quantity of cottonseed meal on the diet for allogynogenetic crucian carp.Experiment one: Apparent digestibility coefficients were determined by using a reference diet with chromic oxide indicator and test diets that contained 70% reference diet , by weight , and 30 % of the test feed ingredient being evaluated. Allogynogenetic crucian carp were fed eleven kinds of diets for 60 days. The fish (averaging 10.1g) were reared in PVC cages. Experiment were conducted to test the effects of test diets on the weight gain; specific growth rates; whole-body; feed efficiency ratio and protein efficiency ratio, and the apparent digestibility of dry matter , crude protein , crude lipid and energy in 10 feed ingredients (fish meal, blood meal, rice bran, soybean meal, Corn gluten meal, peanut meal, barley malt sprouts,peanut cake,rapeseed meal and cottonseed meal). The results showed that, weight gain of allogynogenetic crucian carp ranging from 59.90%~124.36%, specific growth rates of allogynogenetic crucian carp ranging from 0.76%?day-1~1.32%?day-1, apparent digestibility coefficients of dry matter ranging from 55.9% to 89.6% , apparent digestibility coefficients of crude protein ranged from 82.7% to 98.9% indicated that these feed stuffs could be efficiently utilized by allogynogenetic crucian carp. Apparent digestibility coefficients of crude lipid ranging from 69.7%～96.7%,and apparent digestibility coefficients of energy ranging from 62.3%～94.5%.Experiment two : allogynogenetic crucian carp (averaging 19.5g) were fed the diet supplemented with 0%,20%,40%,56% cottonseed meal for 180 days. In order to study supplement the best quantity of cottonseed meal in routine diet. The results indicated that the diet with 40% cottonseed meal had the best weight gain and feed coefficient, quantity of cottonseed meal under 56% had no effect on whole-body, livability, physiological function(superoxide dismutase, SOD; lysozyme, LSZ; gliutamic-pyruvic transminase, GPT; glutamic oxaloacetic transaminase,GOT; hemoglobin, Hb),histology of liver and intestines of allogynogenetic crucian carp. With the quantity of the cottonseed meal increasing, the results of muscle water rose and the results of organs indices reduce. The results showed that the optimal levels of cottonseed meal in diets were 40 %.Experiment three :This study was conducted to evaluate the effect of gossypol on allogynogenetic crucian carp. allogynogenetic crucian carp (averaging 19.5g) were fed the basal diet supplemented with 0 mg/kg,240 mg/kg,480 mg/kg,720 mg/kg gossypol respectively. The trial lasted 180 days. Fish fed the diet containing gossypol from 0 mg/kg to 720 mg/kg ,the growth performance, whole-body, feed efficiency ratio, protein efficiency ratio, muscle water ,organs indices,CF,physiological function(superoxide dismutase, SOD; lysozyme, LSZ; gliutamic- pyruvic transminase, GPT; glutamic oxaloacetic transaminase,GOT; hemoglobin, Hb),histology of liver and intestines have been surveyed. The results showed that the addition of gossypol with 720 mg/kg had no effect on each index surveyed of allogynogenetic crucian carp.