Effects Of Angiogenic Factor On Follicular Angiogenesis And Development In Immature Mouse

Formation of tumor new vessels is controlled by balances between angiogenic stimulators and inhibitors. Angiogenesis play essential function in the follicle development and the selection of ovulated follicles Especially in the maintain of vascular permeability. Therefore,understanding the regulation of angiogenic factor on follicular angiogenesis and development in immature mouse is a very important research task,which provides a powerful help for the artificial control of follicular angiogenesis and the treatment of ovarian dysfunction.This study determined the effects of angiogenic factors (VEGF,FSH,2-ME2,4-OHE2,FSHR antagonist and E2) on follicle numbers, the percentage of atretic follicles and vascular development around the follicle.The results show:(1) FSH and VEGF can increase the weight of ovary,and the effects may be reduced by FSHR antagonist.2-ME2,4-OHE2 and 2-ME2+FSH significantly decreased the ovary weight in immature mouse.(2) Various treatments have a different impact on the number of follicles.The number of large follicles was increased after the injection of FSH and VEGF, however,it was decreased after the injection of 2-ME2,4-OHE2 and VEGF+FSHR antagonisy;The number of medium follicles was increased after the injection of VEGF, FSH had no significant effect, however,it was decreased after the injection of 2-ME2 and E2; The number of small follicles was increased after the injection of 2-ME2 and VEGF, however,it was decreased after the injection of E2.(3) Various treatments have a different impact on the percentage of atretic follicles. There is a increase in the percentage of atretic follicles when the angiogenesis was promoted or inhibited. The percentage of atretic follicles was increased after the injection of 2-ME2 and E2,while FSH inhibited the effect of 2-ME2.(4) Various treatments had no significant effect. Various treatments have a different impact on the angiogenesis Through statistical analyses on the number of red blood cells around the large follicles, FSH and VEGF can promote the angiogenesis,2-ME2 and 4-OHE2 can inhibit the angiogenesis. The promotion effect of VEGF does not depend on FSH.The inhibition of 2-ME2 to the angiogenesis was relieved by FSH. Through statistical analyses on the number of red blood cells around the medium follicles, VEGF can promote the angiogenesis,2-ME2 and 4-OHE2 can inhibit the angiogenesis. The promotion effect of VEGF depend on FSH. The inhibition of 2-ME2 to the angiogenesis was relieved by FSH. Through statistical analyses on the number of red blood cells around the small follicles. Various treatments had no significant effect to the angiogenesis in the small follicles.(5) The othe side of the ovaries was added to detect the expression level of VEGF and Flk-1mRNA. According to our results:VEGF and FSH could promote the expression of the target gene, the promotion effect of VEGF depend on FSH, the promotion effect of FSH could be inhibited by 2-ME2.4-OHE2 and E2 could inhibit the expression level of VEGF.