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Approach On Increasing Germination And Vaccination Rates Of Cynomorium Songaricum Seeds

Posted on:2012-09-21Degree:MasterType:Thesis
Country:ChinaCandidate:R WuFull Text:PDF
GTID:2213330362950064Subject:Resources and utilization of medicinal plants
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This paper chosed a specific root parasitical plant Cynomorium songaricum was employed in the experiment to research the seed coat structure, water absorption laws, the quality diversity in different seed size, inhibitory substances in the seed or not, the germination test of C. songaricum under different treatments and the effect of Nitraria sibirica growth on the C. songaricum vaccination under water control treatments. The purpose is enhancing the germination and vaccination rate of C. songaricum by breaking seed dormancy, as well as providing technical guarantee and theoretical for artificial cultivation of C. songaricum. The main results obtained are as follows:1. Seed size has significant diversity on seed quality indexes, such as thousand seed weight, purity, viability, germination power and germination rate. Except for water content, all the indexes of seed quality were positively correlated with seed size, that means bigger seed size, heavier weight of thousand grains, higher seed viability, higher germination rate and better germination power.2. Water extract of C. songaricum seed obviously inhibit germination and seedling growth of cabbage seeds. In the range of seed tolerable temperature, the inhibitory ability enhanced with increasing seed soaking temperature. The crude extracts under the temperature 45℃showed the strongest inhibitory activity, while the weakest inhibitory activity appeared under 25℃. In the same temperature, the inhibitory activity became weaker with the increase time of extraction.3. Dipping seed in warm water can increase the germination rate of C. songaricum, germination rate of dipping seeds under 45℃for 4 d is the best. Compared with control, the seed germination potential, germination rate and germination index respectively increased by 533.93%, 191.00% and 219.61%.4. Ultrasonic waves can significantly improve the germination rate of C. songaricum. The different frequency and time of ultrasonic wave treatment differ in seed germination potential, germination rate and germination index of C. songaricum, in which ultrasonic frequency 60 Hz, 50 min is the best treatment. Compared with control,seed germination potential, germination rate and germination index increased by 1604.51%, 208.33% and 37.18% respectively. 5. The different water-tempered time have significantly effect on growth indexes of N. sibirica, in which the treatment of water-tempering time for 8 d is the best. Compared with 2 d, the plant of height, stem and leaves of N. sibirica increased by 23.88%,25.37% and 33.33% respectively. The different water-tempered time also significantly impact on physiological indexes of N. sibirica, The internal physiology metabolize changed obviously with different water-tempered time treatment. At the stage of former-term, there is no orderliness about the change of MDA content, proline content and soluble carbohydrate content, but at the stage of latter-term, leaf relative water content and soluble protein content are decreased obviously, while Chl content, MDA content, proline content and soluble carbohydrate content are increased obviously with the lasting of water-tempered time.6. The ratio of sand and soil and water-tempered time significantly effect on vaccination of C. songaricum. With the increasing of sand/soil ratio and water-tempered time, the average vaccination rates of C. songaricum increase firstly and then decrease. The treatment that sand/soil is 1:1 and water-tempered lasts for 8ds shows the highest vaccination rate. Water-tempered last for 8 d, vaccination rates were respectively increased by 58.03%(P<0.01)and 19.34%(P>0.05)than sand/soil ratio of 3:7 and 7:3, and significantly higher than any other treatments.
Keywords/Search Tags:Cynomorium songaricum, Nitraria sibirica, seed germination, vaccination
PDF Full Text Request
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