| This study was conducted on Ctenopharyngodon idellus to study the effects of different contents of Xylooligosaccharide on the ability of Non-specific immune function.1. In the experiment of study on effects of Xylooligosaccharide on the non-specific immunity function, healthy fish with basically the same size and body weight were randomly assigned to five groups, there are three replicates in each group and 35 fish per replicate, four experimental diets A1,A2,A3 and A4 were formulated to contain 1,2,4 and 6 g/kg Xylooligosaccharide respectively, and the control (A0) contained no Xylooligosaccharide. Each group were fed with one of the diets respectively. The influence of Xylooligosaccharide was determined by examining the lysozyme activity, blood serum superoxide dismutase activity and the content of serum complement C3 in Od,7d,14d,28d and 56 d, respectively. The results showed that the immune indexes of experiment groups were markedly enhanced compared with the control in the whole experiment. Among them, the lysozyme activity did not obvious change in group A2, compared with the control. However, the blood serum superoxide dismutase activity and the content of serum complement C3 were significantly higher than the control group(p<0.05), the SOD activity reached to the top level on the 14 d(127.09), the content of serum complement C3 reached to the top level on the 28 d(0.683). Moreover, the lysozyme activity, blood serum superoxide dismutase activity and the content of serum complement C3 in group A3 were significantly higher than the control group (p<0.05), the lysozyme activity reached to the top level on the 28 d(0.165), SOD activity reached to the top level on the 7 d(128.73), the content of serum complement C3 reached to the top level on the 28 d(0.700). These results indicated that Xylooligosaccharide could enhance the non-specific immune function of Ctenophaiyngodon idellus, and the optimum content is 2～4 g/kg.2. In the experiment of study on effects of Xylooligosaccharide on the expression of immune related genes, two experimental diets A2 and A4 were formulated to contain 2 and 4 g/kg Xylooligosaccharide respectively, and the control (A0) contained no Xylooligosaccharide. The expression of three immune related genes in liver, kidney and spleen of interleukin-1β(IL-1β), interferon(IFN) and tumor necrosis factor a(TNFa) were examined by using real-time PCR in 14 d,28 d,42 d and 56 d, respectively. The results showed that the expression of IL-1βin the liver was rising after firstly decreased, however, the expression in the kidney and spleen were significantly increased compared with the control(P<0.05). The expression of IFN in the liver and kidney were firstly rated and then increased, and group A4 was significantly enhanced(P<0.05), The expression of IFN in the spleen of group A2 was not significantly while group A4 was significantly enhanced(P<0.05). The expression of TNFa increased both in the liver and spleen, and it was significantly enhanced(P<0.05) on 28 d while that was on 14 d as well as in the kidney, Somewhat decreased, then after rise. |
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