| The 1000-seed weight and its related traits are important yield traits in Brassica napus which play important roles in seed yield. In this study we use a synthetic material Yunnan061009-1 the 1000-seed weight of which is super large and two small seed materials 07-5189 and 06-1007 to construct F2 segregating populations. Scan the QTLs associate with 3 traits,1000-seed, pod length and seed number per unit length. The main results are showed as follow:1. Compare the parents of two population, it showed that 1000-seed weight, pod length, seed number per unit length are significant different between the large seed material Yunnan061009-1 and two small seed material 07-5189 and Hui 5900.2. Through the phenotype data of F2A and F2B population and normal distribution test of all traits, it showed that 1000-seed weight, pod length, seed number per unit length are normally distribution.,so three traits are all quantitative trait, and F2A and F2B population is suitable for QTL analysis.3. The genetic linkage map of F2A, was constructed with 93 simple sequence repeat (SSR) markers and 135 Amplified fragment length polymorphism (AFLP) markers, which span 1755.25 cM and the average interval distance is 9.50 cM. It has 23 linkage groups. Based on the SSR markers information, we align the map of F2A to the reference maps.4. A linkage map with 127 simple sequence repeat (SSR) markers,52 amplified fragment length polymorphism (AFLP) markers and 24 intron polymorphism (IP) was contructed. It spans 1755.25 cM and has 23 linkage groups.The average interval distance of the linkage group is 9.50 cM. Based on the SSR markers information, we align the map of F2B to the reference maps.5. By composite interval mapping method in Win QTL Cartographer v2.5 software, a total of 9 QTLs in F2A,7 QTLs in F2B associate with 1000-seed weight, pod length and seed number per unit lengthwere found. There is QTLs cluster, but no more effects gene in the result.6. The QTLs qaSW19-2 and qaSW19-3 of F2A population and the QTL qbSW19 are in the same marker interval of BnGMS405-BnGMS371. |
PDF Full Text Request