Somatic Embryogenesis And Plantlet Regeneration In Lilium Leucanthum In 'Qinba' Mountains

'Qinba'Mountains is one of areas with the most plentiful wild lily resources,the area has 11 kinds of wild lily Including variety.In recent years, the environment of the wild lily resources in'Qinba'Mountains has been destroyed by human factors. Population propagation is also infiltrated by external plant species and threated by commercial ball invading.The work of protecting and preservating wild lily resources is very critical. Somatic embryogenesis is one of the in vitro culture of lily methods,which have many advantages such as large quantities,high speed,complete structure and high regeneration rate.This experiment studied tissue culture systerm and somatic embryogenesis systerm of wild lily in'Qinba'Mountains,and researched the factors affected the somatic embryogenesis induced. The main results in this study as follows:1. The experiment of in vitro shoot induction of Lilium leucanthum:The best sterilization time of scales of Lilium leucanthum is: the combination is Alcohol 30s and HgCl2 10min. In the process of disinfection,the pollution rate is 33.33% and the mortality rate is 10%. the best hormonal combination inducing adventitious bud is: MS+1.0mg/L6-BA+0.3mg/LNAA,the induction rate is 86.67%. The ability of different locations in inducing in vitro shoot is :middle>outer>inner.2. The experiment of induction somatic embryogenesis of Lilium leucanthum:The best hormone combination in inducing somatic embryogenesis of three different ecotype of Lilium leucanthum is different. The best hormone combination of the Lilium leucanthum in YuDu,ZhenBa,ShaanXi is: MS+0.5 mg/L TDZ+1.0 mg/L NAA, the induction rate is91.43%. The best hormone combination of the Lilium leucanthum in LiuHou,LiuBa,ShaanXi is: MS+0.5 mg/L TDZ+2.0 mg/LNAA, the induction rate is77.14%. The best hormone combination of the Lilium leucanthum in LiJie,ZhouQu,ShaanXi is: MS+0.5 mg/L TDZ+1.5 mg/LNAA, the induction rate is 85.71%.Dark condition is favorable for inducing somatic embryogenesis of Lilium leucanthum.The ability of different position of scales in inducing somatic embryogenesis of Lilium leucanthum is: down>middle>upper. When sugar concentration of medium is 60g/L, the induction rate is 90% which is the highest.When activated carbon concentration of medium is 0.1 g/L, the induction rate is83.33%which is the highest.3. The best way to establish rapid propagation and regeneration system of Lilium leucanthum:The average number of new bulblet generating by the way of inducing adventitious buds is 4.3. The average number of new bulblet generating by the way of inducing somatic embryogenesis is 11.5.the way of somatic embryogenesis induced is the better way of rapid propagation and regeneration system of Lilium leucanthum:4. The experiment of enlargement,taking root,transplanting of tube plantlet of Lilium leucanthum:The best t hormone combination in enlarging tube plantlet of Lilium leucanthum is : 2MS+0.2 mg/LNAA,Dark condition is favorable for enlarging tube plantlet of Lilium leucanthum. The best hormone combination in taking root of tube plantlet of Lilium leucanthum is: MS+0.2 mg/L NAA+0.1g/LAC,the longest roots is 35.74mm. Dark condition is also favorable for taking root of tube plantlet of Lilium leucanthum.The best acclimatization time of the tube plantlet is 8 days. The survival rate is 83% which is the highest.1/2MS is the best nutrient solution,the survival rate is 80%.When the diameter of bulb is greater than 1cm, The survival rate is highest which is 88%.When the length of root of tube plantlet is between 1cm and 2cm,the survival rate is highest which is 77%.