| In the present study, the common grain feedstuffs (such as corn,wheat,barley and rice) were collected,and their chemical compositions were analysed by the method of proximate analysis.Then the representative samples were screened out based on the ratio of AP/AM of chemical content. The EHGEs (enzymic hydrolyzate gross energy) were analysed by pepsin-artificial small intestinal fluid enzymic method, and their TMEs (true metabolizable energy) were assayed by the method of fasting and force-feeding the adult, male cherry valley ducks. Finally, the effects of the feedstuffs' chemical compositions on their TMEs and EHGEs for ducks were discussed, as well as the correlation between their TME and EHGE.1.23 corn samples were collected and subjected to proximate analysis.As a result, the average ratio of AP/AM is 2.02±0.35. Among them,5 representative samples from the 23 samples were then screened out according to the AP/AM ratio, from lower to higher. They were Heilongjiang corn, Inner Mongolia corn, Dongbei corn, Hubei Hanchuan corn and Hubei Xianning corn, and their AP/AM ratio were 1.4,1.65,1.92,2.31,2.61,respectively. Then, their EHGEs were analysed by enzymic method. At the same time, their TMEs were assayed by the method of fasting and force-feeding the adult, male cherry valley ducks. The correlation between chemical composition and EHGE was assayed, and the regression equation is as follows: EHGE=3.2233Ash+0.2713TS+1.1215GE-26.0720, R2=0.196. Similarly, correlation between Chemical composition and TME was assayed and the regression equation is as follows:TME=-5.7360TS-6.9381AM/AP-0.7105As+90.3668,R2=0.6245. At last, the comparation and correlation between EHGE and TME were analysed. The results showed EHGE was significantly lower than TME (p<0.05), and the regression equation is TME=-0.1589 EHGE+13.207, R2=0.2623.2.17 wheat samples were collected and subjected to proximate analysis.As a result, the average ratio of AP/AM is 3.52±0.73. Among them,5 representative samples from the 17 samples were then screened out according to the AP/AM ratio, from lower to higher. They were Henan wheat, Hubei Macheng wheat, Henan Zhengzhou wheat, Hubei Xiangfan wheat, Hebei Hanchuang wheat, and their AP/AM ratios were 1.84,1.97,2.30, 2.67.3.01, respectively. Then, their EHGEs were analysed by enzymic method. At the same time, their TMEs were assayed by the method of fasting and force-feeding the adult, male cherry valley ducks. The correlation between chemical composition and EHGE was assayed,and the regression equation is as follows: EHGE=0.00720CP+0.30508EE-0.64694Ash+11.5987, R2=0.3097.Similarly,correlation between chemical composition and TME was assayed and the regression equation is as follows:TME=0.4602TS-1.1458AM-1.6457Ash+1.8095, R2=0.3713.At last, the comparation and correlation between EHGE and TME were made.The results showed there was no significant difference between EHGE and TME (P>0.05), and the regression equation is TME=0.6637 EHGE+6.4442, R2=0.3994.3.11 barley samples were collected and subjected to proximate analysis.As a result, the average ratio of AP/AM is 2.29±0.36. Among them,5 representative samples from the 11 samples were then screened out according to the AP/AM ratio, from lower to higher. They were Dongbei barley, Xinjiang barley, Hubei xiangfan barley, Hubei Jingzhou barley,Hubei Xiantao barley, their AP/AM ratios were 2.86,3.10,3.61,3.84. 5.27, respectively. Then, their EHGEs were analysed by enzymic method. At the same time, their TMEs were assayed by the method of fasting and force-feeding the adult, male cherry valley ducks. The correlation between chemical composition and EHGE was assayed, and the regression equation is as follows: EHGE=0.2277AP+0.1597EE-0.1918CP+23.3718, R2=0.3713.Similarly,correlation between Chemical composition and TME was assayed and the regression equation is as follows:TME=0.3079DM-0.0087NDF+0.1272TS-20.0919, R2=0.0823.At last, the comparation and correlation between EHGE and TME were made.The results showed there was no significant difference between EHGE and TME (p>0.05), and the regression equation is TME=0.2831 EHGE+16.384, R2=0.2546.4.8 barley samples were collected and subjected to proximate analysis.As a result, the average ratio of AP/AM is 3.97+-0.97. Among them,5 representative samples from the 8 samples were then screened out according to the AP/AM ratio, from lower to higher. They wereHubei hong'an rice, Guangdong Zhanjiang rice, Hubei Xiangfan rice, Hubei Wuwhan rice, Hubei Huangpi rice, their AP/AM ratios were 2.94,3.28,3.81,4.74,5.00, respectively. Then, their EHGEs were analysed by enzymic method. At the same time, their TMEs were assayed by the method of fasting and force-feeding the adult, male cherry valley ducks. The correlation between chemical composition and EHGE was assayed,and the regression equation is as follows: EHGE=-0.5817CP-0.3579Ash+0.5484NDF+11.5704, R2=0.8357. Similarly,correlation between Chemical composition and TME was assayed and the regression equation is as follows:TME=-0.4721DM+0.6035CP+0.7253Ash+46.2774, R2=0.6298.At last, the comparation and correlation between EHGE and TME were made.The results showed there was no significant difference between EHGE and TME (p>0.05), and the regression equation is TME=-0.2427 EHGE+14.934, R2=0.0693.In summary, conclusions of this study are as follows:The variation patterns of all chemical compositions are different for every grain feedstuff, because of different varieties and maturity.Among them, the coefficient of variation of the ratio of AP/AM is the largest. The total starch content can well predict the true metabolizable energy. Among all the regression model, for corn and rice, the regression models using chemical composition to estimate TME was the best; however, for wheat and barley,the regression model by using emzymatical method to estimate TME was the best. In short, the results can enrich and improve the duck feedstuffs nutrition value data, and provide a theoretical basis for formulating feeds accurately and making scientifical use of grain feedstuffs in practice. |
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