Physiological Responses Of Rat To Spirotetramat And It's Detection Methods

Pesticide is a double edged sword for agriculture except contributed to increase production and income, it also caused some negative effects. Diffusion, residues and enrichment are environmental behavior of chemical pesticides. Chemical pesticide residues can move long distance through air and water and spread from one environmental medium to another, and also affected people and other animals in remote areas through the food chain. Pesticides not only threat to human health through food, water and air but also have some indirect effects on birds, mammals and other higher animals which mainly caused by enriching in the food chain and absorbing from the environment. Currently, the toxicological studies of many types of pesticides have been carried out in amphibians, fish and mammalians. Triazole, amides, nereis toxins and pyrethroids (permethrin bromine fluoride, fluoride cyhalothrin, permethrin, etc.) have been studied more thoroughly. Little reports focused on the toxicology of keto compounds. This paper studied on the physiological responses of rat to spirotetramat and its distribution and residue.1. The UPLC-MS/MS parameters were optimized in spirotetramat analysis method and detecting time was significantly shortened when 0.1% formic acid - acetonitrile was selected as mobile phase, and gradient elution was adopted for the injection method. The efficiency of proton, and adjusting the flow rate and gradient elution conditions was significantly improved through adding the formic acid mobile phase and better separating target peaks was achieved. Only 5 min was taken for analyzing each sample, which could save the analysis time and the reagent consumption. When tuning in the mass spectrometry, a flow injection pump was using for continuous injections, and based on the full scan mode and multiple reaction detection mode (MRM), the m/z374.0/216.0 of spirotetramat was determined as quantitative ion pair, the m/z 374.0/302.0 for qualitative ion pair, the m/z302.3/216.0 for qualitative ion pair.2. The residue detection method was established based on UPLC-MS/MS.Fat, muscle and VisceraI in rat were analyzed respectively, and acetonitrile was used as extraction solvent. The recovery ratios of spirotetramat were 81.6% 103.5% and relative standard deviations within 4.6% 8.9%, The lowest detectable concentration was 0.005 mg / kg. The minimum detectable amount of spirotetramat was 1.2×10^-13g.3. Spirotetramat distributed mainly in liver, kidneys, toxin organs, and detoxification in rat and closed relationship was found between spirotetramat residue and it concentration. when drug concentration was 2 times increased the detection residues is also showed approximate 2 times.4. ACPase activity was strengthened by spirotetramat. Under unorganized body condition, with spirotetramat concentration increased the enzyme activity enhanced, and the both showed line relationship (Y=0.0032X+1.0537,R2=0.9199). Under the intravital food culture condition, spirotetramat 50 mg/kg /d feed for seven days, the enzyme activity was 26% higher than the control group. Spirotetramat was increaseed at 100 mg/kg /d after rising 1.48 times. 7 days after stop drug feeding, ACPase activity reduced to 1.22 times and 1.42 times respectively. 14 days after stop drug feeding, ACPase activity reduced to 1.16 times and 1.36 times respectively.5.Alkaline phosphatase(AKPase) was inhibited by spirotetramat. Under unorganized body condition, with spirotetramat concentration increased the enzyme activity decreased, and the both showed line relationship (Y = 3 X 10^-5X²-0.0044 X + 0.9743.,R² = 0.9449). Under the intravital food culture condition, spirotetramat 50 mg/kg /d feed for seven days, the enzyme activity was 0.94 times of control group. Spirotetramat was increaseed at 100 mg/kg /d the AKPase was 0.861 times of controle in. 7 days after stop drug feeding, ACPase activity increased to 0.96 times and 0.89 times respectively. 14 days after stop drug feeding, ACPase activity increased to 0.97 times and 0.91 times respectively.6. Carboxylesterase(CarE) activity decreased with the spirotetramat concentration increased under unorganized body condition and the both showed line relationship (Y=-0.0013X+0.9639,R²=0.9532).Under the intravital food culture condition, spirotetramat 50 mg/kg /d feed for seven days, the enzyme activity was 0.93 times of control group. Spirotetramat was increaseed at 100 mg/kg /d the AKPase was 0.861 times of controle in. 7 days after stop drug feeding, ACPase activity decreased to 0.96 times and 0.90 times respectively. 14 days after stop drug feeding, ACPase activity increased to 0.97 times and 0.96 times respectively.7. Spirotetramat 50 mg/kg /d and 100 mg/kg /d use rat feeding for 7 days, boday weight and gain utilizing ratio of rat have no significance different . After spirotetramat intake in rat it has striking influence on liver and testicular viscera index and no obviously effect on other organs.