Study On The Pharmacognosy And The Fermentation Process Of Calvatia Gigantean Lloyd

Calvatia gigantea (Batsch ex Pets)Lloyd is the medicinal purposes of fungus of Lycoper-daceae.this thesis determine the pharmacognosy system research of Calvatia gigantean in me-dicinal fungus,and uses the biology fermentation engineering method to develop Calvatia gig-antean the mold mushroom spawn separation to for medicinal purposes of fungus Calvatia gigantea, the purification, the selective breeding, the fermentation process, the chemical composition and the pharmacology activity research, expected to provide the theoretical foundation for the high-quality cultivation and exploitation resources of Calvatia gigantean.Using source identication,character identification,microscopic identification and physical and chemical identification methods to identify gathered of Calvatia gigantean.Applies the organization parting method of the Calvatia gigantea to carry on the separation, the purification and selective breeding, through with the different nutritive elements and the raising condition's inspection breeds selectively vitality, metabolism ability exuberant fine the mold mushroom spawn of the Calvatia gigantean which used in the liquid fermentation technology research. In the liquid fermentation technology research,first to carry on the inspection to the nutrition ingredients of the liquid medium,the nutrition ingredients include the carbon source,the nitrogen source and the Vitamin,then designed the orthogonal test L9 (34) and screened the optimal culture medium;the second to carry on the inspection to culture conditions,selected the optimal culture conditions which were the filling level,the pH value,the temperature and the inoculum concentration;the last to simulates in the big production using the rocker mold mushroom spawn raise to ferment the pot raise, through inspects the laboratory production fermentation raise termination target (hypha wet weight, dry weight,the pH, protein content, polysaccharide content, ergosterol content).That the polysaccharide is the liquid fermentation metabolin,combine with the pH,the mycelial biomass,so selected the 168h-192h was the end of liquid fermentation,when the lovastatin is the liquid fermentation metabolin. selected the 168h was the end of liquid fermentation.Using the UV and the HPLC methods to determine the polysaccharide content and the ergosterol content in the fruiting bodies,liquid fermentation and hyphae of Calvatia gigantean, the results show that the method is simple, accurate and can be used as quality control. This article toevaluate the anti-inflammatory, analgesic and bacteriostatic activity effect of different extracts from the fruiting bodies, the mycelia and the broth of Calvatia gigantean,this three samples has certain analgesic action and anti-inflammation effect and antibacterial, available fermentation metabolites to replace the fruiting bodies of Calvatia gigantean related clinical effect.