Studies On Liquid Culture And Polysaccharides Activity Of Medicinal Mushroom

To investigate the best formula of fermentation medium of three medicinal mushroom by utilizing mycelium as the target product,the Ganoderma lucidum (leyss.:Fr.) Karst,Cordyceps sobolifera (Hill.) Berk. et Br and Laetiporus sulphureus (Fr.) Murrill,which were collected from field acquisition and purification were employed as the experimental materials. Different types of extracting rate of polysaccharides were determined through extracting the polysaccharide of mycelium and fermentation liquid. Furthermore,Lewis lung cancer solid tumors were used to investigate the influencing of polysaccharides to the tumor inhibitory rate. The splenocyte proliferative experiment was used to investigate the stimulatory effects of polysaccharides to the immune cells,in order to exploring the function of different kinds polysaccharides. This can establish scientific basis for the exploring of wild medicinal fungi.Main results are as follows:1.In the appropriate cultural environment,the excellent formula liquidcultural medium for the fungi growth and mycelium production were selected by the comparative experiment of cultural medium formula of medicinal fungi.①The optimum liquid medium of G.lucidum :Glucose 30g,soybean powder 20g,(NH4)2SO4 2g ,KH2PO41g ,MgSO4 0.8g ,VB0.02g,H2O 1000ml。②The optimum liquid medium of C. sobolifera :potato 200g,soybean powder 5g,Glucose 20g,KH2PO4 2g,H2O 1000ml。③The optimum liquid medium of L. sulphureus :Glucose 30g,corn meal 10g,yeast extract 5g ,KH2PO4 1.0g, CaCO3 2.0g , MgSO4 0.5g, H2O 1000ml。2.The polysaccharide extraction of three kinds of mycelium and fermentation liquid were investigated. The results indicated that there were significant differences of the yield rate among distinct medical mycelium and fermentation liquid by the same extracting method. The yield rate of polysaccharide from mycelium and fermentation liquid of G. lucidum was 18.5% and 23.3% respectively and was highest.3.The polysaccharide content and protein content of three medical mushroom were determined. The results shows that the seriatim of the mycelium polysaccharide content is G. lucidum > C. sobolifera > L. sulphureus and the seriatim of the polysaccharide content in Polysaccharide from fermentation liquid is G. lucidum > L. sulphureus > C. sobolifera .4.The inhibition function of three types of medical mycelium to Lewis lung cancer was studied. The results indicated that all of the three types of mycelium polysaccharide can inhibit the tumor. The inhibition ratio of G. lucidum polysaccharide to Lewis lung cancer is highest, which reached to 50.2%. The inhibition ratio of the C. sobolifera polysaccharide and the L. sulphureus polysaccharide was 46.3% and 35.7% respectively.5.The splenocyte proliferation assay showed that the seriatim of the increasing immunity effect of six samples was C. sobolifera polysaccharide of fermentation liquid>C. sobolifera polysaccharide of mycelium >G. lucidum polysaccharide of fermentation liquid >L.sulphureus polysaccharide of fermentation liquid >G. lucidum polysaccharide of mycelium >L. sulphureus polysaccharide of mycelium. The effects of medicinal mushroom polysaccharide of fermentation liquid were better than that of medicinal mushroom polysaccharide of mycelium.