The Research Of Important Rice Resource Molecular Inspection Technology

Rice is the important crops in the gramineous ones,it feeds the most of population in the world.There are abundant germplasm resource in rice,but at present,becase it lacked of corresponding inspection technology and destroyed by people, it causes our rice resources huge losses.It is necessary to protect the rice resource and diversity in order to improve the rice quality and sustainable production. For establishing a rapid and efficient rice resources molecules inspection technology system, this paper collected one hundred and five materials, Itcontains O.rufipogon, O.latiflia, O.alta, O.meyeriana.O.punctata O.officinalis, O.longistaminata, O.eichingeri,eight types of wild rices and two types of cultivated rices. It aimed to established the rice resource inspection technology system for distinguishing the species specificity of wild rice and cultivated rice.1.Using the genetic bar code technology, amplify the rice gene of trnL matK, rpoB, rpoC, Nadl, ndhJ, atpF-atpH, psbK-psb1, Adh2, Os9971,then sequencing,it conducted the system Phylogenetic evolution relation analysi by using MEGA4 for the better sequence result of trnL, rpoB, rpoC, Nadl, ndhJ, atpF-atpH, psbK-psbl and combining with the genetic sequence of genebank published information,the following conclusion (1) trnL had the more polymorphism sites,it can not only distinguish the genetic generic crops in the gramineous ones,but also locate the genomic,it divided the analytical rice material into AA,CC, DD, CCDD four chromosome type. (2) Comparing with other rice material, O.latifolia and O.alta has relative near genetic relationship. (3) atpF-atpH, rpoB, rpoC,ndhJ gene is suit to identify the above of generic crops.2. The dCAPS molecular markers system is established on the basis of the SNP, Nucleotide sequences of eight genes in O. punctata and O.officinalis were analyzed by bio-informatics software to search for SNP loci that are suitable for designing appropriate mismatch primer(s), which allow relevant enzymes to cut on after PCR. In matK gene of two wild rice species DNA, one appropriate SNP loci were found respectively, In trnL gene of O. punctata one appropriate SNP loci were found. on the basis of which four mismatch primer pairs were designed and distinguished O. punctata or O.officinalis from other O.sativa and wild rice after restriction enzyme digestion of PCR products. This result supported that dCAPS molecular markers can be used in port inspection.