Comparative Study On Primary Bioactive Compounds From Green Tea In Shaanxi Province

Green tea has been cultivated for3000years, mainly in China and Southeast Asia. As thefunction of active compounds that were extracted from green tea has been confirmed by alarge number of researches, the consumption of green tea beverage will steady increase in thefuture.The purpose of the research is to determine and compare the contents of Theanine, GallicAcid (GA), Caffeine (CAF), Epicatechin Gallate (EGC), Epigallocatechin Gallate (EGCG)and Epicatechin Gallate (ECG) in the investigated green tea samples from three regions inShaanxi Province by high performance liquid chromatography (HPLC). The results showedthat contents of the six active compounds were in range of0.325~47.061mg/g. By far, thelevel of GA was significantly higher (P<0.05) in Hanzhong green tea, while the levels of theother five active compounds were significantly (P<0.05) higher in Ankang green tea.In addition, the reaserch also determined the protective effects of Theanine on proteindamage and protein carbonyl that were induced by Cu2+/H2O2or AAPH. The results showedthat Theanine could inhibit the protein damage and protein carbonyl that were induced byCu2+/H2O2.Whereas Theanin did not show any protection on protein damage and proteincarbonyl that were induced by AAPH.