Study On Oxidative Damage And Reproductive Genotoxicity Of Formaldehyde In Male Mice

Recently, the problem of indoor air pollution caused by decoration and fitment are following with interests. Because of its characteristics of extensive sources, serious toxicities, high-level pollution and long-term duration, formaldehyde is considered to be one of the most common pollutants. And the study of formaldehyde has become a hot and difficult spot of the environmental and hygienical fields. Our study aims to further calibrate the oxidative damage and reproductive genotoxicity and mechanism of FA inhalation under different level, and to find biomarkers which can be earlier and more conveniently to detect the damage induced by FA.The results:1. Erythrocyte and marrow damage induced by formaldehydeSPF-class Kun-Ming male mice were exposed to gaseous formaldehyde at different concentrations (0.5mg/m~3 and 3mg/m~3) and measure superoxide dismutase activity in erythrocyte and micronucleus rate in bone marrow polychromatic erythrocytes. The result showed 0.5mg/m~3 formaldehyde and 3mg/m~3 formaldehyde both can make superoxide dismutase activity in erythrocyte decrease and micronucleus rate in bone marrow polychromatic erythrocytes increase.2. Oxidative damage induced by formaldehyde in male miceSPF-class Kun-Ming male mice were exposed to gaseous formaldehyde at different concentrations (0.5mg/m~3 and 3mg/m~3 ) and measure total antioxidant capacity, nitric oxide synthase activity, malonyl dialdehyde content and protein carbonyl content to evaluate total antioxidant capacity damage, nitric oxide content, LPO, protein oxidative damage and mechanism induced by gaseous formaldehyde. The result showed 0.5mg/m~3 formaldehyde can make total antioxidant capacity decrease, nitric oxide content and malonyl dialdehyde content increase, but can not induce protein oxidative damage and 3.0mg/m~3 formaldehyde can make total antioxidant capacity decrease, nitric oxide content and malonyl dialdehyde content increase and can induce protein oxidative damage. As gaseous formaldehyde concentration increased, the damage was moreseverity.3. Reproductive genotoxicity of formaldehyde in male miceIn this study micronuclei test and comet assay were applied to detect reproductive genotoxicity induced by formaldehyde in male mice. The result showed that gaseous formaldehyde could increase micronucleus rate in early stage sperm cells and formaldehyde induced DNA breaks at 10nmol/L, 25umol/L and 50jimol/L, DNA breaks and DNA cross-links at 75[imol/L, Tail DNA% and Tail moment had no significant difference between formaldehyde group of lOOjxmol/L and control group, and the critical concentration at which formaldehyde can induced DNA strand breaks of testicular cells was about lOfimol/L, peak point was near 50u,mol/L. This study offers certain theoretical foundation for the molecular mechanism of reproductive toxicity by formaldehyde.