Analysis And Determination Of Several Kinds Of Compounds In Tobacco Flavor

The application of tobacco flavor is closely related to the development of cigarette brand. So the analysis and quality control of tobacco flavor has very important significance. In this paper several constituents in tobacco flavor were studied by continuous flow analyzer(CFA), ultra performance liquid chromatography(UPLC), ion chromatography(IC) and inductively coupled plasma mass spcctromctry(ICP-MS). The main work of this thesis is summarized as follows1.Twelve kinds of tobacco flavor samples were extracted with5%acetic acid water solution. The contents of total sugar, reducing sugar, nicotine and chlorine were determined by CFA in the same time. The results indicated that the recoveries of total sugar, reducing sugar, nicotine, chlorine were from93.60%to107.80%and the RSDs were from1.18%to4.89%.2. A method for simultaneous determination of osthole and columbianadin in tobacco flavor by UPLC was established.20kinds of tobacco flavor samples were extracted by acetionitrile-water (volume ratio of1:1) separated at30℃on ACQUITY UPLC(?)BEH C18(2.1mm i.d×50mm1.7μ.m) column eluted with acetonitrile-water as the mobile phase. The flow rate was set at0.30mL/min and the detected wavelength was set at324nm. The results indicated that the chromatographic peak of osthole and columbianadin standard samples were sharp and symmetric. The resolution of this two substances were greater than1.5. The calibration curve of osthole and columbianadin were linear within the range from0.20to10.00mg/L(r=0.9999).20kinds of tobacco flavor were not detected containing osthole and columbianadin. The blank samples were added osthole and columbianadin. The average recoveries of osthole were from98.00%to100.44%and the RSDs were from0.74%to2.54%in three days. The average recoveries of columbianadin were from99.41%to101.02%and the RSDs were from0.92%to2.27%in three days.3. A method for determination of imperatorin in tobacco flavor by UPLC was established.20kinds of tobacco flavor samples were extracted by acetionitrile-water (volume ratio of1:1) separated at30"Con ACQUITY UPLC(?)BEH C,18(2.1mm i.d×50 mm1.7μm) column eluted with acetonitrile-water as the mobile phase. The flow rate was set at0.20mL/min and the detected wavelength was set at248nm. The results indicated that the chromatographic peak of imperatoin standard samples were sharp and symmetric. The calibration curve of imperatoin was linear within the range from0.16to8.00mg/L(r=0.9999). The RSDs of imperatoin were from0.68%to2.97%in three days and the average recoveries were from90.83%to97.85%.4. A method for determination of theobromine in tobacco flavor by UPLC was established.20kinds of tobacco flavor samples were extracted by water separated at30℃on ACQUITY UPLC(?)BEH C18(2.1mm i.d×50mm1.7μm) column eluted with methanol-water as the mobile phase. The flow rate was set at0.10mL/min and the detected wavelength was set at274nm. The results indicated that the chromatographic peak of theobromine standard samples were sharp and symmetric. The calibration curve of theobromine was linear within the range from0.16to8.00mg/L(r=0.9998). The RSDs of theobromine were from0.14%to1.58%in three days and the average recoveries of theobromine were from91.86%to93.70%.5. A rapid IC combined liquid-phase-extraction method was developed for the separation and determination of7anions in tobacco flavor.10m mol/L sodium hydroxide solution was used to directly extract the anions from tobacco flavor. The results indicated that the recoveries of anions were from90.48%to98.42%and the RSDs were from0.69%to1.25%.6. A method for the determination of24kinds of trace elements in tobacco flavor by ICP-MS with microwave digestion was studied. In this paper,8kinds of tobacco flavor samples were determined. The Ge and Th were added as internal standard to eliminate the matrix interferences and using the shrub standard material for method validation. The results indicated that the detection limilt of24kinds of trace elements were from0.004%to1.078%and the RSDs were less than10%and the average recoveries were from84.46%to108.85%.