Studies On The Application Of Sequential Injection Technique In The Analysis Of Phenolic Organic Pollutants

Polluants increased remarkably with the fast increase of population and development of economy. Organic pollutants attracted the most serious primary focus due to its high toxicity, hard degradable and bioaccumulative. It is very important for the environmental protection to detect pollutions quickly and precisely. Sequential injection analysis(SIA) was introduced as an automatic analytical technique at the beginning of 90s last century. The attractive features of SIA as an analytical tool are simplicity,very low consumption of samples and reagents and simultaneous determination of multi-components. It is also a green analytical technique. Studies on the application of SIA in the analysis of phenolic organic pollutants were carried out in this thesis. The paper consists of five chapters.In chapter 1, the principle and apparatus of sequential injection analysis were introduced. The current development SIA was reviewed in the analysis of organic pollutants.In chapter 2, a rapid method was developed for the determination of resorcinol by sequential injection analysis. It is based on the inhibitory effect of resorcinol on the reaction rate between KBrO3 and Rhodamine B in acid solution. Under the optimum experimental conditions, the linear range for resorcinol is 0.20³.00μg mL^-1, and the detection limit is 0.09μg mL^-1. Up to 40 samples can be analyzed per hour. The method has been applied to determine resorcinol in synthetic samples and environmental water samples, the results were compared with those obtained by high performance liquid chromatography. Statistical analysis revealed that there was no significant difference at 95 % confidence level.In chapter 3, a rapid and sensitive sequential injection spectrophotometric method for determination of bisphenol A was described. The method was based on the inhibitory effect of BPA on the reaction between methylene blue and KBrO3 in acidic medium. The linear range of BPA was 0.1～2.0μg mL^-1 and the detection limit of 0.05μg mL^-1 was achieved with a sample throughout of 45 h-1. The method was applied to the determination of BPA in environmental water samples, and the results were statisfactorily compared with the values obtained by high performance liquid chromatography.In chapter 4, based on the inhibitory effect of hydroquinone on the reaction of Safranine T with KBrO3 in acidic medium, a rapid and simple sequential injection spectrophotometric method for determination of hydroquinone was described. Under the optimum experimental conditions, the linear range of the calibration curve for hydroquinone was 0.05～1.50μg mL^-1 and the detection limit of 0.02μg mL^-1 was achieved. The total reagent consumption in a cyclical procedure was only 125μL. The method was used to determine hydroquinone in tap water, surface water and river water. The results were statisfactorily compared with the values obtained by HPLC.In chapter 4, the main innovative contents concerned in this paper were summarized, and the future work has been suggested.